品牌与标准化Issue(5)：4-6,10,4.DOI:10.3969/j.issn.1674-4977.2024.05.002
超高效液相色谱法测定消炎止咳胶囊中槲皮素和山柰素的含量
Determination of Quercetin and Kaempferol in Xiaoyan Zhike Capsules by UPLC
摘要
Abstract
Objective:To develop a UPLC method for simultaneous determination of quercetin and kaempferol in Xiaoyan Zhike Capsules and enhancing the quality control standard of the preparation.Methods:The UPLC content determination method is employed.Chromatographic conditions:BEH C18(2.1 mm×150 mm,1.7 μm)column is used for separation;acetonitrile-0.4 phosphoric acid solution(25∶25)served as the mobile phase;the detection wavelength is 370 nm;the column temperature is 30℃;the flow rate is 0.3 mL/min,and the injection volume is 2 μL.Results:The linear ranges of quercetin is 1.7365～17.3620 μg/mL(R2=0.9999,n=5);the recoveries are 99.5%with an RSD of 1.0%.The linear ranges of kaempferol is 2.4516～24.5154 μg/mL(R2=0.9999,n=5);the spiked recoveries are 98.2%and the RSD is 1.2%.Conclusion:A UPLC method for determining quercetin and kaempferol in Xiaoyan Zhike capsules is established,effectively contributing to the quality control of this preparation.关键词
消炎止咳胶囊/胡颓子叶/槲皮素/山柰素(酚)/超高效液相色谱Key words
Xiaoyan Zhike capsules/folium elaeagni/quercetin/kaempferol/UPLC引用本文复制引用
刘志海,彭啟丽,王丹丹,金昭..超高效液相色谱法测定消炎止咳胶囊中槲皮素和山柰素的含量[J].品牌与标准化,2024,(5):4-6,10,4.基金项目
贵州省2022年度省级药品探索性研究课题. （）
