热带作物学报2024，Vol.45Issue(8)：1659-1669,11.DOI:10.3969/j.issn.1000-2561.2024.08.015
香蕉枯萎病4株生防菌株的诱变改良及抑菌机制初探
Mutation Improvement of Four Biocontrol Strains and the Inhibition Mechanism Against Banana Fusarium Wilt
摘要
Abstract
Banana is an important economic crop in the subtropical region of China.The occurrence of banana Fusarium wilt has seriously hindered the development of banana industry in recent years.Biological control has attracted attention because of its green environmental protection and durable disease resistance.It's very important to get the efficient biocontrol strains with different inhibition mechanisms on banana Fusarium wilt.In this study,four different types of biocontrol strains against banana Fusarium wilt which were obtained in the laboratory in the early stage were mutated by ultraviolet-nitrite compound mutagenesis,with the highest antimicrobial activity increased by 186.24％and the highest control effect increased by 46.54％.The disease prevention mechanism of the four mutants obtained was pre-liminarily investigated.The results showed that the strain Ba62v had the highest inhibition rate on the colony growth of Fusarium oxysporum f.sp.cubense(Foc),and could also induce the activity of SOD enzyme in banana seedlings.The strain Blz02v could promote the growth of banana seedlings and induce the activities of POD and SOD of banana seed-lings.The strain Bc11v had the highest inhibition rate on the spore germination of Foc,and could induce the activities of PPO,CAT and SOD in banana seedlings.The strain Pt05v had the highest inhibition rate on the sporulation of Foc,and could induce the activities of POD,PPO and SOD in banana seedlings.The main action mechanisms of the four biocon-trol mutants on Foc and hosts are different,and there is a potential for biocontrol of compound synergism,which would provide an important strain resource for the development and utilization of the disease-resistant compound fungicides in the future,and has a good application prospect.关键词
香蕉枯萎病/生物防治/诱变改良/生防机制Key words
banana Fusarium wilt/biological control/mutation improvement/biocontrol mechanism分类
农业科技引用本文复制引用
杨迪,杜婵娟,张晋,潘连富,蒋尚伯,李春雨,邓国仙,付岗..香蕉枯萎病4株生防菌株的诱变改良及抑菌机制初探[J].热带作物学报,2024,45(8):1659-1669,11.基金项目
广东省基础与应用基础研究基金项目(No.2020B1515420006) （No.2020B1515420006）
中央引导地方科技发展资金项目(桂科ZY21195015) （桂科ZY21195015）
国家自然科学基金项目(No.31560006). （No.31560006）
