热带作物学报2024，Vol.45Issue(8)：1677-1684,8.DOI:10.3969/j.issn.1000-2561.2024.08.017
苏铁羽叶褐斑病病原菌鉴定及其化学防治药剂的筛选
Identification of Pathogen Causing Brown Spot in Leaves of Cycas and Its Chemical Control Agent Screening
摘要
Abstract
This research aimed to identify a pathogenic fungus of the brown spot disease in the leaves of Cycas and screen for its chemical control agents,which provides a theoretical basis for the protection of Cycas.The pathogenic fungus was isolated from the lesion spots in some Cycas leaves.The pathogenicity of the fungus was confirmed by iso-lating and purifying the infected leaf tissues and reintroduced to the healthy leaves of Cycas diannanensis following Koch's postulates.Morphological identification of the pathogenic fungus was conducted.To further confirm the identi-fication,a phylogenetic tree was constructed using eight gene sequences:SSU,LSU,GAPDH,tef1,rpb2,Alt,OPA10-2 and ITS.Effective chemical control agents were screened from seven chemical agents by inhibition assay in vitro.Phy-logenetic analyses showed that this pathogen was Alternaria burnsii.Among the tested chemical agents,the most effec-tive inhibition was fluoxastrobin,which displayed 73.48％inhibition rate at a concentration of 0.67 mg/L.关键词
苏铁/褐斑病/病原鉴定/巴斯基链格孢/平板抑菌试验Key words
Cycas/brown spot/pathogen identification/Alternaria burnsii/the inhibition assay in vitro分类
农业科技引用本文复制引用
覃茜,丁丽琼,池昭锦,陆祖正,单彬,谢振兴,黄歆怡,许恬..苏铁羽叶褐斑病病原菌鉴定及其化学防治药剂的筛选[J].热带作物学报,2024,45(8):1677-1684,8.基金项目
南宁植物园横向项目业务费专项(No.2020QXS-YLB-FW01) （No.2020QXS-YLB-FW01）
广西农业科学院基本科研业务专项(桂农科2021YT152). （桂农科2021YT152）
