四川环境2024，Vol.43Issue(4)：164-170,7.DOI:10.14034/j.cnki.schj.2024.04.022
产纤维素酶菌株的筛选、鉴定及产酶特性的研究
Study on Screening,Identification and Enzyme Production Characteristics of Cellulase-producing Strains
摘要
Abstract
Cellulose,as a main constituent of various plant straws,widely exists in nature,and cellulase,an enzyme that catalyzes the degradation of cellulose to produce glucose,can be used in different ways to reduce the enrichment of cellulose in the environment.In order to obtain cellulose-degrading strains with high enzyme-producing capacity,a strain with high cellulase-producing capacity,CTE1-1,was isolated and screened from humus using the Congo red plate method and identified as Bacillus sp.Through the one-factor experiments of incubation time,incubation temperature,initial pH and NaCl concentration,it was determined that the bacterium was cultured in enzyme-producing medium containing 1％CMCA-Na at 30℃,initial pH 7.0 and NaCl concentration 1.5％,and the enzyme activity of CMCA enzyme could be up to 42.59 U/mL after 108h incubation,and the enzyme activity of FPA enzyme could be up to 45.66 U/mL after 96h incubation.The cellulase produced by this strain showed strong enzyme activity at pH 6.0 and 50℃,Fe3+and K+had activation ability for the cellulase produced by this strain,Mg2+had no effect on the cellulase produced by this strain,and Cu2+and Zn2+had certain inhibitory ability for it.This strain has a strong ability to produce cellulase and can be used for the production of cellulase preparations.rain has a strong ability to produce cellulase and can be used for the production of cellulase preparations.关键词
纤维素酶/芽孢杆菌/酶活力Key words
Cellulase/Bacillus sp./enzyme activity分类
资源环境引用本文复制引用
张富强,孙劲,张文丽,陈梦越,黄诗维..产纤维素酶菌株的筛选、鉴定及产酶特性的研究[J].四川环境,2024,43(4):164-170,7.基金项目
凉山州科技计划(21ZDF0181) （21ZDF0181）
西昌学院"两高"人才科研支持计划项目(LGZ202001) （LGZ202001）
四川省大学生创新训练项目(S202210628045) （S202210628045）
四川省大学生创新训练项目(S202310628086). （S202310628086）
