水产学报2024，Vol.48Issue(9)：124-135,12.DOI:10.11964/jfc.20220613527
基于环境DNA metabarcoding的中街山列岛鱼类物种多样性
Fish species diversity in the Zhongjieshan Islands based on environmental DNA metabarcoding
摘要
Abstract
Rocky reef habitats are critical for supporting exceptionally high fish species diversity,a vital element of biodiversity.However,monitoring fish species in these habitats using traditional fisheries resource survey meth-ods poses significant challenges.Therefore,there is an urgent need to develop sensitive and efficient methods for biodiversity monitoring.Four sampling stations were strategically selected around the Miaozihu Islands within the Zhongjieshan archipelago,and surface seawater samples were collected across four seasons(February,May,August and November in 2019).The spatial and temporal distribution of fish was analyzed using environmental DNA metabarcoding technology.The findings revealed a total of 37 fish species across 10 orders 26 families 36 genera.The seasonal abundance of fish species followed a trend of summer＞winter＞autumn＞spring.Remark-ably,only 2 fish species were observed in all four seasons,representing approximately 5.41％of the total species count,while 54.05％of the species were detected in a single season only.The non-metric multidimensional scal-ing(NMDS)analysis and the analysis of similarities(ANOSIM)test indicated significant variation in fish compos-ition among seasons(PP＜0.05),but not among stations.The fish community's uniformity remained relatively stable.Although the diversity and richness indices did not exhibit significant differences among stations,they peaked in summer and troughed in spring.Overall,the distribution of fish in the coastal waters of the Zhongjie-shan Islands is significantly influenced by the seasonal changes rether than station locations.This study introduces an innovative,environmentally friendly approach to fish diversity assessment in marine environments akin to those of the Zhoushan islands and reefs,offering valuable technical insights for the management and conservation of fisheries resources.关键词
鱼类/环境DNA metabarcoding/物种多样性/中街山列岛Key words
fish/environmental DNA metabarcoding/species diversity/Zhongjieshan Islands分类
农业科技引用本文复制引用
钟兰萍,高天翔,张浩博,陈治,王晓艳..基于环境DNA metabarcoding的中街山列岛鱼类物种多样性[J].水产学报,2024,48(9):124-135,12.基金项目
浙江省重点研发计划(2021C02047) （2021C02047）
国家自然科学基金(41806180) Zhejiang Provincial Key Research and Development Program(2021C02047) （41806180）
National Natural Science Foundation of China(41806180) （41806180）
