miR-27a-3p在肺腺癌中的表达及其生物学功能OACSTPCD

Objective:The aim of this study was to detect the expression level of miR-27a-3p in lung adenocarcinoma(LUAD),and study the effect of miR-27a-3p on the malignant growth and migration of LUAD cells.Methods:The expres-sion level and prognostic value of miR-27a-3p in LUAD were analyzed by integrating public datasets.Inhibitor was transfect-ed to decrease the level of miR-27a-3p in LUAD cells.Cell proliferation activity was detected by EdU,cell migration was de-tected by Transwell chamber,and the level of miR-27a-3p and its targeted gene were detected by real-time fluorescent quan-titative PCR.Results:The expression of miR-27a-3p was significantly high in LUAD(P＜0.05),and the expression level of miR-27a-3p was increased in cancer tissue of young LUAD patients compared with other age groups.The prognosis of LUAD patients with high expression of miR-27a-3p was poor.miR-27a-3p inhibitors remarkably attenuated cell proliferation activity and cell migration ability in LUAD(all P＜0.05).Combined with public data and cell experiments,our results showed that RELN,NCALD,FZD4 and GATA2 might be downstream target genes of miR-27a-3p in LU AD.In addition,the expressions of RELN,NCALD,FZD4 and GATA2 were significantly low in LUAD(all P＜0.05).Conclusion:High expres-sion of miR-27a-3p promotes the malignant growth and metastasis of LUAD cells through targeting RELN,NCALD,FZD4 and GATA2.