肿瘤预防与治疗2024，Vol.37Issue(8)：641-648,8.DOI:10.3969/j.issn.1674-0904.2024.08.002
miR-27a-3p在肺腺癌中的表达及其生物学功能
Expression and Biological Functions of miR-27a-3p in Lung Adenocarcino-ma
摘要
Abstract
Objective:The aim of this study was to detect the expression level of miR-27a-3p in lung adenocarcinoma(LUAD),and study the effect of miR-27a-3p on the malignant growth and migration of LUAD cells.Methods:The expres-sion level and prognostic value of miR-27a-3p in LUAD were analyzed by integrating public datasets.Inhibitor was transfect-ed to decrease the level of miR-27a-3p in LUAD cells.Cell proliferation activity was detected by EdU,cell migration was de-tected by Transwell chamber,and the level of miR-27a-3p and its targeted gene were detected by real-time fluorescent quan-titative PCR.Results:The expression of miR-27a-3p was significantly high in LUAD(P＜0.05),and the expression level of miR-27a-3p was increased in cancer tissue of young LUAD patients compared with other age groups.The prognosis of LUAD patients with high expression of miR-27a-3p was poor.miR-27a-3p inhibitors remarkably attenuated cell proliferation activity and cell migration ability in LUAD(all P＜0.05).Combined with public data and cell experiments,our results showed that RELN,NCALD,FZD4 and GATA2 might be downstream target genes of miR-27a-3p in LU AD.In addition,the expressions of RELN,NCALD,FZD4 and GATA2 were significantly low in LUAD(all P＜0.05).Conclusion:High expres-sion of miR-27a-3p promotes the malignant growth and metastasis of LUAD cells through targeting RELN,NCALD,FZD4 and GATA2.关键词
miR-27a-3p/肺腺癌/细胞迁移/细胞增殖Key words
miR-27a-3p/Lung adenocarcinoma/Cell migration/Cell proliferation分类
生物科学引用本文复制引用
牛静静,张文桃,畅靖嘉,张昕彤,朱剑军..miR-27a-3p在肺腺癌中的表达及其生物学功能[J].肿瘤预防与治疗,2024,37(8):641-648,8.基金项目
中国博士后科学基金(编号:2023M732157) （编号:2023M732157）
山西省基础研究计划项目(编号:202303021211114) （编号:202303021211114）
山西医科大学校级博士启动基金(编号:XD1808) This study was supported grants from China Postdoctoral Science Foundation(No.2023M732157),Science and Technology Department of Shanxi Province(No.202303021211114),and Shanxi Medical University(No.XD1808). （编号:XD1808）
