肿瘤预防与治疗2024，Vol.37Issue(8)：649-657,9.DOI:10.3969/j.issn.1674-0904.2024.08.003
铁死亡相关基因影响肝细胞癌肿瘤微环境及预后风险研究
Effects of Ferroptosis-Related Genes on Microenvironment and Prognosis in Hepatocellular Carcinoma
摘要
Abstract
Objective:To explore the heterogeneity of hepatocellular carcinoma(HCC),and investigate the association between ferroptosis-related genes and tumor microenvironment(TME),then construct a prognosis prediction model based on these genes through bioinformatics methods.The model aims to offer new strategies for preventing recurrence in HCC pa-tients.Methods:Based on TCGA and NODE databases,non-negative matrix factorization clustering was applied to identify molecular subtypes of ferroptosis-related genes.The ESTIMATE and CIBERSORT algorithms were employed to calculate im-mune scores,while Cox regression analysis was used to develop a risk assessment model and calculate risk scores.A nomo-gram incorporating risk scores and clinical features was established,and in vitro experiments were conducted to validate mR-NA expression levels.Results:HCC was classified into two distinct ferroptosis-related immune molecular subtypes,showing significant difference in immune scores(P＜0.001).G6PD,KIF20A,RRM2 and TXN were identified as the optimal can-didate genes for the risk assessment model.The nomogram accurately predicts the likelihood of HCC recurrence.In vitro ex-periments revealed increased expression levels of these four genes in liver cancer cells(P＜0.05).Conclusion:HCC can be classified into two molecular subtypes which are associated with HCC recurrence,suggesting a critical role of ferroptosis in the TME.The four identified genes may be potential factors for preventing HCC recurrence,and the prognosis prediction model shows significant potential for predicting recurrence of HCC.关键词
铁死亡/肝细胞癌/肿瘤微环境/无复发生存期/生物信息学Key words
Ferroptosis/Hepatocellular carcinoma/Tumor microenvironment/Recurrence-free survival/Bioinformatics分类
医药卫生引用本文复制引用
肖芳平,汪文华,贾菠,罗勇兵,熊莉娟,吴冬梅,周银平..铁死亡相关基因影响肝细胞癌肿瘤微环境及预后风险研究[J].肿瘤预防与治疗,2024,37(8):649-657,9.基金项目
江西省科技创新平台基地组建项目(编号20212BCD46003) This study was supported by grants from Science and Technology Department of Jiangxi Province(No.20212BCD46003). （编号20212BCD46003）
