拟南芥损伤诱导的钙信号传递模式的研究OACSTPCD

In order to investigate the generation and transmission of systemic calcium signals in plants,this study used different damage methods,such as chemical damage and mechanical damage.The GCaMP6s fluorescent indicator,which can record the real-time changes in free calcium ion concentration in the cytoplasm,was used to observe and compare the changes in calcium ion signals produced by Arabidopsis thaliana leaves after stimulation.The long-distance transmission patterns of calcium ion signals induced by different damages in the plant were systematically recorded and analyzed.The results showed that adding water droplets to the surface of Arabidopsis thaliana leaves would cause relatively weak calcium signal production,which was transmitted along the developmental sequence of the leaves.The calcium signals induced by chemical damage and mechanical damage stimuli are mainly generated by damaged tissues and transmitted to adjacent leaves.When using strong acids for chemical damage and cutting leaves for mechanical damage,the long-distance transmission speed of calcium ion signals in plants is faster than other methods.Different damage signals lead to differences in the speed,intensity,and mode of long-distance transmission of calcium signals in plants,indicating that plants adopt diverse long-distance signal transmission methods to adapt to environmental changes.