首都师范大学学报（自然科学版）2024，Vol.45Issue(4)：77-84,8.DOI:10.19789/j.1004-9398.2024.04.010
液相色谱质谱联用分离鉴定酸性植物激素的初级教学实验
Elementary teaching experiment on the separation and identification of acidic plant hormones by LC-MS
摘要
Abstract
Liquid chromatography-mass spectrometry(LC-MS)is currently one of the important technical means for the detection of plant hormones such as abscisic acid(ABA)and indole-3-acetic acid.In order to consolidate students'grasp of the basic concepts and principles of LC-MS,improve their practical abilities and cultivate their experimental literacy,this paper provides 5 basic teaching experiments,ranging from easy to difficult,to address the main problems in current domestic LC-MS experimental teaching.These experiments can help students understand the basic process of LC-MS sample pretreatment,recognize the complexity of real samples and the bottleneck issues in the determination of trace plant hormones and learn preliminarily the method for the calculation of ABA content in real samples.After consecutive practice for three semesters in the Instrument Analysis course for the first year graduate students,these five teaching experiments have shown their teaching efficiency to cultivate the ability for students to use modern large-scale analytical instruments and to enhance their interest to learn instrument analysis technology.关键词
教学实验/脱落酸/吲哚乙酸/液相色谱质谱联用仪/定性检测Key words
teaching experiment/abscisic acid/indole-3-acetic acid/liquid chromatography-mass spectrometry(LC-MS)/qualitative detection分类
农业科技引用本文复制引用
徐奭,盛仙永,崔素霞..液相色谱质谱联用分离鉴定酸性植物激素的初级教学实验[J].首都师范大学学报（自然科学版）,2024,45(4):77-84,8.基金项目
国家自然科学基金面上项目(31970296) （31970296）
