陕西中医2024，Vol.45Issue(9)：1155-1160,6.DOI:10.3969/j.issn.1000-7369.2024.09.001
温阳健脾方调节AMPK/mTOR/ULK1通路上调RSA小鼠孕期自噬水平改善其蜕膜化的机制研究
Study on the mechanism of Wenyang Jianpi decoction regulating AMPK/mTOR/ULK1 pathway,up-regulating autophagy level in RSA mouse during pregnancy and improving decidualization
摘要
Abstract
Objective:To study the influence of Wenyang Jianpi decoction on autophagy level of recurrent spon-taneous abortion(RSA)mice during pregnancy by influencing AMPK/mTOR/ULK1 signal axis.Methods:5 CBA/J female rats and BALB/c male rats were caged to establish 10 normal control rats.CBA/J female mice were caged with DBA/2 male mice to establish RSA mouse model,which were randomly divided into model group and middle dose group of Wenyang Jianpi decoction,with 10 mice in each group.From the first day of pregnancy,the Wenyang Jianpi decoction group was given a moderate dose of Wenyang Jianpi decoction 13.96 g/(kg·d),while the control group and the model group were given the same volume of normal saline.After 14 days of continuous intervention,eyeball blood was taken,decidual tissue was taken,the embryo loss rate was calculated,and insulin-like growth factor-1(IGFBP-1),Prolactin(PRL)and HE staining were used to observe the morphology of mouse endometrium,and Western blot was used to determine the expressions of AMPK,p-AMPK,p-mTOR,p-ULK1,LC3Ⅱ/LC3Ⅰ,Beclin1 and p62 in mouse decidua.Results:Compared with the control group,the embryo absorption rate of RSA model group decreased significantly(P<0.01).Serum IGFBP-1 and PRL decreased,the relative expression of LC3Ⅱ/LC3Ⅰ pro-tein decreased significantly(P<0.001),the relative expression of p-AMPK/AMPK and Beclin1 protein decreased significantly,and the expression levels of mTOR,ULK1 and p62 protein increased significantly(P<0.01).Com-pared with the model group,the embryo absorption rate of Wenyang Jianpi decoction group decreased significantly(P<0.05).The levels of serum IGFBP-1 and PRL increased(P<0.05),while the relative expression levels of LC3Ⅱ/LC3Ⅰ,p-AMPK/AMPK and Beclin1 all increased,while the relative expression levels of mTOR,ULK1 and p62 decreased(P<0.05).Conclusion:Wenyang Jianpi decoction can promote the autophagy level of RSA mice during pregnancy through AMPK/mTOR/ULK1 pathway,thus improving decidualization and effectively increasing the pregnancy rate.关键词
复发性流产/自噬/蜕膜化/温阳健脾方/AMPK/mTOR/ULK1信号通路/阴阳平衡/胰岛素样生长因子结合蛋白1/催乳素Key words
Recurrent spontaneous abortion/Autophagy/Decidualization/Wenyang Jianpi decoction/Signaling pathway of AMPK/mTOR/ULK1/The balance of yin and yang/Insulin-like growth factor-1/Prolactin分类
医药卫生引用本文复制引用
赵贵霜,刘娟,李孟瑶,姜丽娟..温阳健脾方调节AMPK/mTOR/ULK1通路上调RSA小鼠孕期自噬水平改善其蜕膜化的机制研究[J].陕西中医,2024,45(9):1155-1160,6.基金项目
国家自然科学基金地区科学基金资助项目(82060882) （82060882）
