肾衰泄浊汤对腺嘌呤致肾阳虚型慢性肾衰竭大鼠下丘脑-垂体-肾上腺轴的影响OACSTPCD

Objective:To evaluate the effects of Shenshuai Xiezhuo decoction on indexes related to kidney-yang deficiency and hypothalamic-pituitary-adrenal(HPA)axis of chronic renal failure(CRF)induced by adenine gavage model.Methods:SD rats were randomly divided into normal group,model group,low-dose,medium-dose,high-dose Chinese medicine group and western medicine group with 10 rats each.The Kidney-Yang deficiency CRF rat model was established by adenine administration.At the end of modeling,the low,medium and high concentration groups were given different concentrations of Shenshuai Xiezhuo decoction(9.375,18.75 and 37.5 g/kg),losartan potassi-um was used as positive control,and normal group was given normal saline once a day for 28 consecutive days.The general activities of rats were observed,and the indexes of body weight,urine volume and water intake were recor-ded.The contents of Scr and BUN in serum were detected.The pathological changes of renal tissue were observed by HE staining.The levels of CRH,ACTH and CORT in serum of rats were determined by ELISA.Results:Shenshuai Xiezhuo decoction could improve the performance of crouching back,cold back,slow crawling,slow reaction and slow growth of body mass,and reduce water and urine volume related to kidney-yang deficiency in CRF rats.The patho-logical destruction of renal tissue was alleviated and serum Scr and BUN levels were decreased.The contents of CRH,ACTH and CORT in serum were increased.Conclusion:Shenshuai Xiezhuo decoction can alleviate the related manifestations of kidney-yang deficiency syndrome in adenine-induced CRF rats,reduce the content of Scr and BUN in ser-um,alleviate the pathological injury of kidney tissue,and increase the levels of CRH,ACTH and CORT in HPA axis.