白虎加人参汤对2型糖尿病大鼠抗氧化应激作用及机制研究OACSTPCD

Objective:To study the effect and mechanism of Baihu jia Renshen decoction(BHJRS)on oxidative stress in Type 2 diabetic rats,and to explore its mechanism.Methods:Eight SD rats were randomly selected as the control group(NC group),and the remaining SD rats were treated with high fat and high sugar combined with low dose of STZ(45 mg/kg)intraperitoneally to create T2DM rat model.The model rats were randomly divided into a model(DM)group,a positive drug[metformin(Met),200 mg/kg group],a BHJRS high-dose group(BHJRS-H,37.8 g/kg),a BHJRS low-dose group(BHJRS-L,18.9 g/kg).The drug was administered continuously for 8 weeks.FBG was measured at the 0,2,4,6 and 8 weeks in each group,and the levels of HbAlc,INS,MDA,GSH-Px,SOD and CAT were detected,and ISI values were calculated.The morphological changes of pancreatic histopathology were observed.The mRNA and protein levels of Bcl-2,Bax and Caspase-3 were detected in pancreatic tissue.The expres-sion of AKT/GSK-3β/Nrf2 signaling pathway in pancreatic tissue was detected.Results:Compared with DM group,the levels of FBG,serum HbAlc and MDA in Met group,BHJRS-H group and BHJRS-L group were significantly de-creased(P<0.05),and the levels of INS,CAT,SOD,GSH-Px and ISI in serum were significantly increased(P<0.05).Significantly increased the p-Akt/AKT ratio,p-GSK-3β/GSK-3β ratio,n-Nrf2 and heme oxygenase-1(HO-1)lev-els in pancreatic tissue(P<0.05),and significantly decreased the Bax/Bcl-2 ratio,Caspase-3 and n-Fyn levels(P<0.05).The histopathologic changes of pancreas were improved in different degree.Conclusion:BHJRS has a cer-tain effect on the anti-oxidative stress of pancreatic tissue,and its mechanism may be related to the regulation of the expression of related proteins in the AKT/GSK-3β/Nrf2 signaling pathway.