陕西中医2024，Vol.45Issue(9)：1172-1175,1185,5.DOI:10.3969/j.issn.1000-7369.2024.09.004
雷公藤甲素调控NLRP3/GSDMD/IL-1β途径改善六价铬对足细胞损伤的机制研究
Study on the mechanism of triptolide regulating NLRP3/GSDMD/IL-1β pathway to improve podocyte injury induced by Cr(Ⅵ)
摘要
Abstract
Objective:To explore the protective effect of triptolide(TP)on podocytes by regulating NLRP3/GS-DMD/IL-1β pathway by establishing a mouse podocyte injury model.Methods:Mouse podocyte MPC5 was cultured and Cr(Ⅵ)podocyte injury model was established.The mice were divided into normal group(Control group),Cr(Ⅵ)group and Cr(Ⅵ)+TP group(TP group).The effect of 30～150μmol/L Cr(Ⅵ)on podocyte viability and the effect of 2～10 ng/ml TP on podocyte treated by Cr(Ⅵ)were detected by CCK-8 method to determine the best concentration and time of Cr(Ⅵ)and TP intervention.The expression of NLRP3,Caspase-1,GSDMD,IL-1β and IL-18 scorch related protein was detected by Western blot method,and the expression of WT-1 protein was detected by cellular immunofluorescence.Results:The results of CCK assay showed that the cells were treated with Cr(Ⅵ)at 150μmol/L for 1 h and TP at 2 ng/ml for 24 h.The results of Westernblot showed that the expression of NLRP3,Caspase-1,GSDMD,IL-1β and IL-18 in Cr(Ⅵ)group was higher than that in Control group(P<0.05,P<0.01).The expression of NLRP3,Caspase-1,GSDMD,IL-1β,and IL-18 was decreased in the TP group(P<0.05).The re-sults of cellular immunofluorescence showed that the positive expression of WT-1 in Cr(Ⅵ)group was significantly lower than that in Control group.The expression was elevated after the TP intervention.Conclusion:TP can improve the injury effect of Cr(Ⅵ)on podocytes by inhibiting NLRP3/GSDMD/IL-1β signal pathway.关键词
雷公藤甲素/六价铬/足细胞/NLRP3炎症小体/慢性肾脏病/细胞焦亡Key words
Triptolide/Cr(Ⅵ)/Podocyte/NLRP3/Chronic kidney disease/Pyroptosis分类
医药卫生引用本文复制引用
魏晓菲,王晨丹..雷公藤甲素调控NLRP3/GSDMD/IL-1β途径改善六价铬对足细胞损伤的机制研究[J].陕西中医,2024,45(9):1172-1175,1185,5.基金项目
山西省科技厅自然科学研究面上项目(202103021224382) （202103021224382）
中国医师协会循证医学专业委员会肾科学组"PRO·润"基金资助项目(KYS2021-03-02-10) （KYS2021-03-02-10）
