陕西中医2024，Vol.45Issue(9)：1176-1180,5.DOI:10.3969/j.issn.1000-7369.2024.09.005
补阳还五汤通过调控miR-210减轻大鼠脑缺血再灌注损伤的实验研究
Experimental study on reducing cerebral ischemia-reperfusion injury in rats by regulating miR-210 with Buyang Huanwu decoction
摘要
Abstract
Objective:To investigate whether Buyang Huanwu decoction can regulate B lymphoblastoma-2-asso-ciated X protein(Bax),B lymphoblastoma-2(Bcl-2)and Caspase-3 in cerebral cortex by inhibiting micro RNA-210(miR-210),thereby alleviating ischemia reperfusion injury(CIRI)in rats.Methods:100 SD rats were randomly divid-ed into 5 groups,and focal cerebral ischemia reperfusion(MCAO)model was established in model group and Buyang Huanwu decoration group,sham operation was performed in sham operation group.The low,middle and high dose groups of Buyang Huanwu decoction were given 200 mg/kg,300 mg/kg and 400 mg/kg of Buyang Huanwu decoc-tion,respectively,and the sham operation group and model group were given the same volume of normal saline for continuous perfusion for 7 days.Neurological deficit score,cerebral infarction rate,cerebral water content and expres-sion of miR-210,Bax,Bcl-2 and Caspases-3 in cerebral cortex were compared among all groups.Results:Compared with the model group,the neurological deficit score of high-dose Buyang Huanwu decoration group was decreased af-ter 1 day of intragastration(P<0.05).After 3 days of intragastric administration,compared with model group and low dose group,the neurological deficit score of medium and high dose groups decreased,and compared with model group,high dose group's neurological deficit scores also decreased(P<0.05).After 7 days of inadministration,there were statistical significance in neural function deficit score,cerebral infarction rate,cerebral water content,miR-210,Bax,Bcl-2 and Caspase-3 between the model group and Buyang Huanwu decoration groups,and between Buyang Huanwu decoration group and Buyang Huanwu Tang group(P<0.01).Conclusion:Buyang Huanwu decoction can regulate the expression of apoptosis-promoting/apoptosis-suppressing genes in the cerebral cortex by inhibiting miR-210,thus alleviating the neurological damage in CIRI rats,and the highest dose has the best efficacy.关键词
缺血性脑中风/脑缺血再灌注/微小RNA-210/补阳还五汤/神经功能/细胞凋亡Key words
Ischemic stroke/Cerebral ischemia reperfusion/MicroRNA-210/Buyang Huanwu decoction/Nerve function/Apoptosis分类
医药卫生引用本文复制引用
张建平,王文,屈阳柳,李剑波,胡瑞斌,崔涛,石军..补阳还五汤通过调控miR-210减轻大鼠脑缺血再灌注损伤的实验研究[J].陕西中医,2024,45(9):1176-1180,5.基金项目
陕西省中医药管理局科研项目(SZY-NLTL-2022-032) （SZY-NLTL-2022-032）
