陕西中医2024，Vol.45Issue(9)：1181-1185,5.DOI:10.3969/j.issn.1000-7369.2024.09.006
黄芪多糖通过TGF-β1/Smads通路对哮喘大鼠Th1/Th2免疫失衡的调节作用
Effects of Astragalus polysaccharide on Th1/Th2 immune imbalance in asthmatic rats through TGF-β1/Smads pathway
摘要
Abstract
Objective:To investigate the astragalus polysaccharides by transforming growth factor TGF(trans-forming growth factor)-β1/Smads signal pathway pathway to asthma rats helper T cells(T helper cell,Th)1/Th2 immune imbalance adjustment.Methods:Forty male SD rats were selected and divided into control group,model group,Astragalus polysaccharide low-dose,medium-dose and high-dose groups(8 rats each group).The rat model of chronic asthma was established,and rats in the control group and model group were injected with equal volume of normal saline through tail vein,and tetramethylpyrazine was injected intraperitoneally at low,medium and high do-ses.All groups were treated continuously for 14 days.After intervention,the damaged bronchial mucosa area and smooth muscle thickness of rats in each group were detected,bronchoalveolar lavage fluid(BALF)was collected,and the changes of interleukin-4(IL-4)and interferon-γ(IFN-γ)levels were measured,and the changes of lung tissue structure were observed by hematoxylin-eosin(HE)staining.Real-time quantitative fluorescent PCR(qRT-PCR)and Western blot were used to determine the mRNA and protein expressions of TGF-β1,Smad2 and Smad3 in lung tissues.Results:Compared with the control group,the damaged area of bronchial mucosa and the thickness of smooth muscle in the model group were increased,and compared with the model group,the astragalus polysaccharide in each group were decreased,in a dose-dependent manner(P<0.05).Compared with the control group,IFN-γ level in BALF of the model group was decreased and IL-4 level was increased;compared with the model group,IFN-γ level in BALF of all Astragalus polysaccharide groups was increased and IL-4 level was decreased in a dose-dependent man-ner(P<0.05).The model group had significantly higher mRNA and protein expressions of TGF-β1,Smad2,and Smad3 in lung tissue than the control group,and the APS groups had significantly lower mRNA and protein expres-sions of TGF-β1,Smad2,and Smad3 than the model group in a dose-dependent manner(P<0.05).Conclusion:As-tragalus polysaccharide can inhibit the activation of TGF-β1/Smads signal to prevent airway inflammation in asth-matic rats,regulate the imbalance of Th1/Th2 ratio,and improve the pathological injury of lung tissue in rats.关键词
黄芪多糖/哮喘/转化生长因子β1/辅助性T细胞/TGF-β1/Smads信号通路/Th1/Th2Key words
Astragalus polysaccharide/Asthma/Transforming growth factor β1/T helper cells/TGF-β1/Smads signaling pathway/Th1/Th2分类
医药卫生引用本文复制引用
王媛媛,王桂玲,耿雨作,杨江..黄芪多糖通过TGF-β1/Smads通路对哮喘大鼠Th1/Th2免疫失衡的调节作用[J].陕西中医,2024,45(9):1181-1185,5.基金项目
江苏省中医药科技发展计划面上项目(MS2022132) （MS2022132）
江苏省宿迁市自然科学基金资助项目(K202111) （K202111）
