上海农业学报2024，Vol.40Issue(4)：60-66,7.DOI:10.15955/j.issn1000-3924.2024.04.10
淡紫拟青霉及哈茨木霉混配冻干粉剂的制备及药效研究
Preparation and efficacy of lyophilized power of Paecilomyces lilacinus and Trichoderma harzianum
摘要
Abstract
This study focused on Paecilomyces lilacinus and Trichoderma harzianum as the research subjects.The objective was to select a suitable lyophilized protective agent and concentration for the preparation of lyophilized powder of Paecilomyces lilacinus and Trichoderma harzianum.Additionally,we conducted an indoor in vitro test using the bacterial agent mixture,targeting control of Botryosphaeria berengeriana f.sp.Piricola.The results showed that the optimal formulation for the Paecilomyces lilacinus lyophilized protective agent consisted of 10％sucrose,10％lactose,and 5％glycerol.When pre-frozen at-80 ℃ for 18 hours,the Paecilomyces lilacinus lyophilized survival rate was 85.23％with a bacterium-to-protectant ratio of 2∶3.The lyophilized protective agent for Trichoderma harzianum contained 15％sucrose,2％peptone,and 5％glycerol.When pre-frozen at-80 ℃ for 18 hours,the survival rate of Trichoderma harzianum following lyophilization reached 95.25％when the bacterium-to-protective agent ratio was 1∶1.Furthermore,when the lyophilized powder of Trichoderma harzianum and Paecilomyces lilacinus were mixed in a ratio of 6∶4,it demonstrated the most effective prevention against Botryosphaeria berengeriana f.sp.Piricola,with a remarkable prevention and control efficiency of 70.24％.These findings addressed the challenges associated with freeze-dried fungicides,such as easy inactivation,short survival time in the environment,and unstable efficacy.Moreover,they provided a solid foundation for the development of novel and effective biopesticide formulations,marking a significant milestone in promoting the growth of the biopesticide industry.关键词
冻干粉剂/淡紫拟青霉/哈茨木霉/苹果轮纹病Key words
Lyophilized power/Paecilomyces lilacinus/Trichoderma harzianum/Botryosphaeria berengeriana f.sp.Piricola分类
农业科技引用本文复制引用
王凯,周桐,李洋洋,杨大杰,殷志鹏,何璐..淡紫拟青霉及哈茨木霉混配冻干粉剂的制备及药效研究[J].上海农业学报,2024,40(4):60-66,7.基金项目
辽宁省民生科技计划项目(2021JH2/10200016) （2021JH2/10200016）
