上海农业学报2024，Vol.40Issue(4)：74-80,7.DOI:10.15955/j.issn1000-3924.2024.04.12
黟县黑猪微卫星遗传多样性分析
Microsatellite analysis of genetic diversity of Yixian black pigs
摘要
Abstract
The blood samples of 55 Yixian black pigs were selected in this experiment,and 14 microsatellite markers were typed by PCR(Polymerase chain reaction)and PAGE(Polyacrylamide gel electrophoresis)to analyze the genetic diversity of Yixian black pig population.The results showed that a total of 171 alleles were detected at 14 microsatellite loci in Yixian black pig population,with an average of about 12 alleles per locus.The number of effective alleles was 3.11-12.37,and the observed heterozygosity ranged from 0.31 to 1.The polymorphism content of 14 microsatellite loci was 0.65-0.91,all of which were highly polymorphic,and the cumulative exclusion probability was 99.9％,which indicated that the selected 14 microsatellite markers could identify the genetic relationship of Yixian black pigs.关键词
黟县黑猪/微卫星标记/遗传多样性/聚丙烯酰胺凝胶电泳Key words
Yixian black pig/Microsatellite marker/Genetic diversity/PAGE分类
农业科技引用本文复制引用
李雪婷,董林,王重龙,刘林清,张威,苏世广,周学利,周梅..黟县黑猪微卫星遗传多样性分析[J].上海农业学报,2024,40(4):74-80,7.基金项目
安徽省财政农业种质资源保护与利用资金项目 （）
安徽省农业科学院重点实验室项目(2021YL023) （2021YL023）
安徽省学术和技术带头人后备入选项目(2022H300) （2022H300）
安徽省自然科学基金青年基金项目(2108085QC135) （2108085QC135）
安徽省生猪产业技术体系项目(AHCYJSTX-05-12、AHCYJSTX-05-23) （AHCYJSTX-05-12、AHCYJSTX-05-23）
六安市产学研合作重大专项(2020年) （2020年）
