上海农业学报2024，Vol.40Issue(4)：86-91,6.DOI:10.15955/j.issn1000-3924.2024.04.14
超高效液相色谱-串联质谱法同时测定玉米和小麦中玉米赤霉烯酮和玉米赤霉烯酮-14-葡萄糖苷检测方法的建立
Development of a UHPLC-MS/MS method for simultaneous determination of zearalenone and zearalenone-14-glucoside in maize and wheat
摘要
Abstract
To scientifically control and ensure food quality and safety,maize and wheat were used as materials,extracted with acetonitrile/water/formic acid solution,purified by HLB solid-phase extraction column,and accurately quantified by matrix matching standard solution,and a reliable ultra-high performance liquid chromatography-mass spectrometry(UHPLC-MS/MS)method was established for simultaneous determination of zearalenone(ZEN)and zearalenone-14-glucoside(ZEN-14G)in maize and wheat.The results showed that ZEN and ZEN-14G exhibited a good linear relationship in the range of 0.1-200 μg/L(R2＞0.999),with a detection limit of 0.03-0.3 µg/kg and a quantification limit of 0.1-1.0 pg/kg.At three levels of addition of 20,50 μg/kg,and 100 µg/kg,the recovery rate was(80.2±9.8)％—(98.6±4.2)％,and the precision was 0.2％—9.1％(n=5).This method is accurate,highly sensitive,and can meet the detection requirements of ZEN and ZEN-14G in maize and wheat.关键词
玉米赤霉烯酮/玉米赤霉烯酮-14-葡萄糖苷/超高效液相色谱-串联质谱法/玉米/小麦Key words
Zearalenone/Zearalenone-14-glucoside/UHPLC-MS/MS/Maize/Wheat分类
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蔡亚玲,张志岐,董芳,聂冬霞,韩铮,李志忠,赵志辉..超高效液相色谱-串联质谱法同时测定玉米和小麦中玉米赤霉烯酮和玉米赤霉烯酮-14-葡萄糖苷检测方法的建立[J].上海农业学报,2024,40(4):86-91,6.基金项目
上海市科技兴农技术创新项目(2020-02-08-00-12-F01453) （2020-02-08-00-12-F01453）
上海市农业科学院卓越团队项目[沪农科卓(2022)0011] （2022）
