上海海洋大学学报2024，Vol.33Issue(5)：1074-1083,10.DOI:10.12024/jsou.20231104350
马氏珠母贝L-氨基酸氧化酶基因克隆及表达分析
Cloning and expression analysis of L-amino acid oxidase gene in Pinctada fucata martensii
摘要
Abstract
L-amino acid oxidase(LAAO)is a kind of immune protease widely found in nature.In order to explore the gene sequence characteristics of LAAO(PmLAAO)and its expression changes after stimulation by Vibrio parahaemolyticus,the full-length cDNA of PmLAAO was cloned in this study.The length of its open reading frame(ORF)is 1767 bp,encoding a total of 588 amino acids,and has the structure domain of FAD-binding domain and amino acid oxidase(Amino_oxidase),which is a member of the amino acid oxidase family.The results of multiple sequence comparisons and phylogenetic trees showed that PmLAAO was closely related to bicrustacea,among which LAAO was the most similar to the Mytilus californianus.qRT-PCR results showed that PmLAAO was expressed in gill,mantle,adductor muscle,gonadal gland and digestive gland,with the highest expression level in gill tissue.After stimulation by Vp,the expression level of PmLAAO in gill tissue was significantly increased,and the expression level was the highest 48 h after stimulation.These results provide a reference for the functional activity of the novel immune protease LAAO in bivalve shellfish.关键词
马氏珠母贝/L-氨基酸氧化酶/基因克隆/实时荧光定量PCRKey words
Pinctadafucata martensii/L-amino acid oxidase/doing/real time quantitative PCR分类
农业科技引用本文复制引用
罗贝,林海生,王庆恒,秦小明,曹文红,高加龙,郑惠娜..马氏珠母贝L-氨基酸氧化酶基因克隆及表达分析[J].上海海洋大学学报,2024,33(5):1074-1083,10.基金项目
广东省自然科学基金(2021A1515012137) （2021A1515012137）
国家现代农业产业技术体系项目(CARS-49) （CARS-49）
广东海洋大学博士启动项目(R17082) （R17082）
广东省现代农业产业技术体系创新团队项目(2021KJ146) （2021KJ146）
广东省高等学校科技创新团队项目(2021KCXTD021) （2021KCXTD021）
