上海海洋大学学报2024，Vol.33Issue(5)：1098-1105,8.DOI:10.12024/jsou.20240504546
NaHCO3胁迫对草鱼鳃和肝胰脏组织抗氧化酶与组织结构的影响
Effects of NaHCO3 stress on antioxidant enzymes and tissue structure in gill and hepatopancreas of grass carp
摘要
Abstract
In order to explore the effects of NaHCO3 stress on antioxidant enzymes and tissue structure of gill and hepatopancreas of grass carp,three NaHCO3concentration groups(10,20,30 mmol/L)were used to stress grass carp with NaHCO3,and the antioxidant enzymes(SOD,CAT)activity and histopathological observation of gill and hepatopancreas were carried out.The results showed that under NaHCO3 stress,the activities of SOD and CAT in gill and CAT in hepatopancreas of grass carp increased first and then decreased within 72 h,and only the activity of SOD in hepatopancreas increased within 72 h.In each experimental group,the activities of antioxidant enzymes in the two tissues were significantly different from those in the blank group.Compared with the blank group,in the gill tissue of grass carp in the experimental group,the gill filaments were seriously folded and wrinkled,the PVC of the flat epithelial cells was seriously exfoliated,and the MRC of the mitochondria-enriched cells was swollen;in the hepatopancreas,the swelling of the pancreatic cells,the increase of the intercellular space,the deviation of the hepatocyte nucleus and the decrease of the vacuolization were observed.Studies have shown that NaHCO3 stress can affect the antioxidant level of grass carp and cause tissue damage to the gills and hepatopancreas of grass carp,and the degree of damage increases with the increase of concentration.This study could provide reference for the breeding and application of grass carp in saline-alkali waters.关键词
草鱼/NaHCO3胁迫/鳃和肝胰脏组织/组织病理学/抗氧化酶Key words
grass carp/NaHCO3stress/gill and hepatopancreas tissue/histopathology/antioxidant enzymes分类
农业科技引用本文复制引用
陈立伟,姜雨辰,贾学文,李家乐,邱军强,沈玉帮..NaHCO3胁迫对草鱼鳃和肝胰脏组织抗氧化酶与组织结构的影响[J].上海海洋大学学报,2024,33(5):1098-1105,8.基金项目
国家现代农业产业技术体系项目(CARS-45-04) （CARS-45-04）
