上海海洋大学学报2024，Vol.33Issue(5)：1106-1119,14.DOI:10.12024/jsou.20231104347
水体中添加乳酸钙对中华绒螯蟹生长、蜕壳、钙沉积和水质指标的影响
Effects of adding calcium lactate to water on growth,molting,calcium deposition of Chinese mitten crab and water quality
摘要
Abstract
In order to study the effects of calcium lactate to water on the growth,molt,and calcium deposition of juvenile Chinese mitten crab(Eriocheir sinensis),this study conducted a 90-day(two complete molting cycles)rearing experiment on female juvenile crabs with an initial body mass of(4.78±0.51)g.Calcium lactate was added to freshwater at concentrations of 0 mg/L,218 mg/L,436 mg/L and 654 mg/L and the calcium ion content in water is 45.6 mg/L,84.4 mg/L,123.2 mg/L and 163.6 mg/L.(recorded as Treatment 1# to 4#).The results showed:(1)The addition of calcium lactate improved survival rates of juvenile E.sinensis significantly.(2)With the increase in calcium lactate content,there was an upward trend of body mass,weight gain rate,specific growth rate,and hepatopancreas index of juvenile E.sinensis during the first molting cycle,but the differences were not significant.However,during the second molting cycle,the body weight,carapace length and carapace width growth rates of juvenile E.sinensis significantly increased with the calcium lactate addition,and among them,treatment 3# and 4# were significantly higher than treatment 1#.(3)The addition of calcium lactate had no significant effect on the intermolt duration but significantly reduced the rate of limb loss after each molting.(4)In terms of calcium deposition,the addition of calcium lactate significantly increased the calcium content in hemolymph during the first and second molting cycles,as well as calcium content in the muscle during the first molting cycle.(5)Increasing calcium lactate content significantly elevated the expression of es EcR2-mRNA and es RXR-mRNA.(6)Calcium lactate had no significant effect on water pH,ammonia nitrogen and nitrite,but water hardness and alkalinity significantly rose with increasing calcium lactate content.In summary,the addition of 654 mg/L calcium lactate to the water improved the survival,growth performance,calcium deposition in tissues,and expression of molting-related genes in juvenile E.sinensis,as well as reduced the limb loss rate after each molting.关键词
中华绒螯蟹/乳酸钙/生长性能/蜕壳/钙沉积Key words
Eriocheir sinensis/calcium lactate/growth performance/molting/calcium deposition分类
水产学引用本文复制引用
黄俊杰,朱筛成,马天利,蒋栋梁,成永旭..水体中添加乳酸钙对中华绒螯蟹生长、蜕壳、钙沉积和水质指标的影响[J].上海海洋大学学报,2024,33(5):1106-1119,14.基金项目
财政部和农业农村部国家产业技术体系建设项目(CARS-48) （CARS-48）
