上海海洋大学学报2024，Vol.33Issue(5)：1120-1131,12.DOI:10.12024/jsou.20231204363
不同低盐驯化方式对缢蛏行为及生理的影响
Behavioral and physiological responses of Sinonovacula contricta to different low-salt domestication patterns
摘要
Abstract
In order to study the optimal low salt acclimation method of Sinonovacula contricta,the low salt semi-lethal salinity was set by 5 gradients of salinity 0,1,2,3 and 5,and the low salt semi-lethal salinity was 2.005 at 96 h.With this salinity as the target salinity,three different domestication methods A,B and C were set(Group A:equal-velocity domestication;Group B:alternate domestication;Group C:gradient acclimation).The S.constricta were acclimated with low salinity from 16 to 2,and then acclimated to the target salinity for 4 days.The survival rate and submerged sludge rate of three acclimation methods were recorded.Oxygen consumption rate and ammonia emission rate of each group were detected.Superoxide dismutase(SOD)activity and Malondialdehyde(MDA)content were determined.The results showed that the survival rate(88％ )and submerged mud rate(90％ )of S.constricta in group C were significantly higher than those in groups A and B when acclimated to salinity 2.The oxygen consumption rate and ammonia emission rate of group C were significantly higher than those of groups A and B.Superoxide dismutase(SOD)activity reached its peak at salinity 4,and group C was significantly higher than groups A and B.Malondialdehyde(MDA)content reached its peak when acclimated to salinity 2,and was significantly lower in group C than in groups A and B.In conclusion,the low salt acclimation method of gradient acclimation(C)group is more beneficial to low salt culture of S.constricta.The results of this study provide an important reference for the development of Marine shellfish aquaculture species in China's inland low-salt waters.关键词
缢蛏/驯化/低盐/行为/生理Key words
Sinonovacula constricta/domestication/low salt/behavior/physiology分类
农业科技引用本文复制引用
杨栋,韩雨婷,高葛琪,王杰,牛东红..不同低盐驯化方式对缢蛏行为及生理的影响[J].上海海洋大学学报,2024,33(5):1120-1131,12.基金项目
国家重点研发计划(2023YFD2401002) （2023YFD2401002）
国家自然科学基金(32373118) （32373118）
