同济大学学报（医学版）2024，Vol.45Issue(4)：488-495,8.DOI:10.12289/j.issn.2097-4345.23212
miR-1290对非酒精性脂肪性肝病的调控机制研究
Regulatory mechanism of miR-1290 in non-alcoholic fatty liver disease
摘要
Abstract
Objective To explore the role and potential regulatory mechanism of miR-1290 in non-alcoholic fatty liver disease(NAFLD).Methods GenBank GEO database,Targetscan,mirBase,miRanda biological databases were screened to find out miRNAs abnormally expressions in NAFLD,and GO and KEGG analysis target gene prediction databases were used to select miRNAs related to GH-IGF1 axis.NAFLD was induced in HL-7702(L02)cells to establish L02 NAFLD cell line.L02 cells(control group)and NAFLD cells were treated with 25,250 ng/mL rhGH and 50,500 ng/mL rhIGF1,respectively;and the expression of miRNAs was detected with RT-qPCR and verified by cell transfection.Results In NAFLD group,the expressions of miR-1290,miR-16,miR-122 were significantly higher than those in control(P＜0.05);after treatment of rhGH and rhIGF1 the expression levels in NAFLD group were decreased,compared to those in control group(P＜0.05).In L02 NAFLD cells transfected with mimics miR-1290,the expression levels of GHR,IGFBP3,FASN mRNA were significantly higher than those in control group(P＜0.05).Conclusion MiRNAs are abnormally expressed in NAFLD,miR-1290 has higher expression in NAFLD cells,which may act on lipid metabolism and insulin resistance in NAFLD through GH/IGF1 axis.关键词
非酒精性脂肪性肝病/miRNAs/胰岛素抵抗/脂代谢/GH/IGF1轴Key words
non-alcoholic fatty liver disease/miRNAs/insulin resistance/lipid metabolism/GH/IGF1 axis分类
医药卫生引用本文复制引用
邹琳,孙菲,李映璇,陈霞,马俊花..miR-1290对非酒精性脂肪性肝病的调控机制研究[J].同济大学学报（医学版）,2024,45(4):488-495,8.基金项目
上海市浦东新区卫生系统优秀青年医学人才培养计划(PWRq2020-30) （PWRq2020-30）
上海市卫生健康委员会项目(202040315) （202040315）
上海市浦东新区公利医院青年英才基金(GLRq2018-02) （GLRq2018-02）
