世界中医药2024，Vol.19Issue(15)：2213-2221,9.DOI:10.3969/j.issn.1673-7202.2024.15.001
基于网络药理学和实验研究小续命汤治疗中风的药效物质及作用机制
Pharmacodynamic Substances and Mechanism of Xiaoxuming Decoction in the Treatment of Stroke Based on Network Pharmacology and Experimental Verification
摘要
Abstract
Objective:To explore the effective substances and mechanism of Xiaoxuming Decoction(XXMD)in the treatment of stroke.Methods:UHPLC-Q Exactive Plus HRMS was used to identify the components of XXMD.Network pharmacology was em-ployed to predict the active components and potential mechanism of XXMD in treating stroke.The quail embryo chorioallantoic membrane(qCAM)experiment was used to visualize the angiogenic effect of XXMD.Results:Sixty-eight components were identi-fied from XXMD,including amino acids,alkaloids,flavonoids,coumarins,terpenes,and butylphenyl peptides,such as ephedrine,cimicifugoside,and baicalin.A protein-protein interaction(PPI)network was constructed based on intersection targets of compo-nents and stroke,with 33 core targets identified,including IL-6,TNF,EGFR,PDGFRB,and PIK3CA.Fifty-seven active components were identified,including ephedrine,baicalin,and ginsenoside Rg1.Enrichment analysis of the core targets suggested that the treat-ment of stroke by XXMD may primarily involve pathways related to angiogenesis,such as the phosphatidylinositol 3-kinase/protein kinase B(PI3K-AKT)and vascular endothelial growth factor(VEGF)signaling pathways.In the qCAM experiment,the positive control group(Gas,0.02 mg)and the high-dose XXMD group(XXMD-H,2 mg,equivalent to 16.69 mg of crude drug)showed a significant increase in the relative vascular area compared to the blank group(P＜0.05),effectively visualizing the angiogenic effect of XXMD.Conclusion:This study elucidated the effective substances of XXMD in treating stroke by combining UHPLC-Q Exactive Plus HRMS and network pharmacology methods.The potential mechanism may be related to promoting angiogenesis through pathways involving PI3K-AKT and VEGF.The qCAM experiment further evaluated its angiogenic activity,and RT-qPCR confirmed related targets,providing a reference for the quality research and further development of XXMD.关键词
小续命汤/超高效液相色谱/高分辨质谱/网络药理学/中风/血管生成/磷脂酰肌醇3-激酶/蛋白激酶B/血管生成因子Key words
Xiaoxuming Decoction/UHPLC/HRMS/Network pharmacology/Stoke/Angiogenesis/PI3K-AKT/VEGF分类
医药卫生引用本文复制引用
张佟,卢继辉,戴子琦,林怡萱,马涛,徐冰,雷海民..基于网络药理学和实验研究小续命汤治疗中风的药效物质及作用机制[J].世界中医药,2024,19(15):2213-2221,9.基金项目
国家自然科学基金项目(82104365,82274082) （82104365,82274082）
河北省自然科学基金项目(H2022329003) （H2022329003）
