世界中医药2024，Vol.19Issue(15)：2260-2264,5.DOI:10.3969/j.issn.1673-7202.2024.15.007
枳术郁李方对脾-肠气机升降失调便秘小鼠的通便机制研究
Laxative Mechanism of Zhizhu Yuli Formula on Constipation Mice with Spleen-intestine Qi Dysfunction in Ascending and Descending
摘要
Abstract
Objective:To investigate the laxative mechanism of Zhizhu Yuli Formula on constipation mice with spleen-intestine qi dysfunction in ascending and descending based on the theory of qi movement,providing experimental evidence for its clinical appli-cation.Methods:Male Kunming mice were randomly divided into a blank group,a model group,a positive drug group,and a Zhizhu Yuli Formula group,with 10 mice in each group.The positive drug group received Maren soft capsule suspension at 0.1 g/kg,the Zhizhu Yuli Formula group received Zhizhu Yuli Formula solution at 0.483 g/kg,and the blank and model groups received an e-quivalent volume of solvent.After 14 days of continuous administration,the mice were given the modeling drug loperamide hydro-chloride(4 mg/kg)via gavage to establish a constipation model with spleen-intestine qi dysfunction in ascending and descending.The small intestine ink propulsion rate,the time to the first black stool,the number and weight of black stools within 6 hours,and the levels of serum vasoactive intestinal peptide(VIP),5-hydroxytryptamine(5-HT),substance P(SP),nitric oxide(NO),motilin(MTL),cholecystokinin(CCK),peptide YY(PYY),and calcitonin gene-related peptide(CGRP)were measured.Results:Com-pared with the model group,the Zhizhu Yuli Formula group showed a significant increase in small intestine ink propulsion rate(P＜0.01),a significant reduction in the time to the first stool(P＜0.01),and a significant increase in the number,weight,and water content of fecal pellets within 6 hours(P＜0.01).Moreover,the serum levels of VIP,NO,CCK,and PYY were significantly reduced(P＜0.01),while the levels of SP,CGRP,and MTL were significantly increased(P＜0.01).Conclusion:Zhizhu Yuli Formula has a significant laxative effect,and its mechanism may be related to regulating the spleen-intestine qi dysfunction in ascending and de-scending,restoring the dynamic balance of gastrointestinal neurotransmitters and hormones,regulating qi movement,and maintaining smooth qi flow.关键词
枳术郁李方/便秘/气机升降/胃肠激素/神经递质/活性肠肽/胃动素/小鼠Key words
Zhizhu Yuli Formula/Constipation/Qi movement/Gastrointestinal hormones/Neurotransmitter/Vasoactive intestinal peptide/Motilin/Mice分类
医药卫生引用本文复制引用
贾岚,刘佳琪,扈觐玺,冯颖童,刘双巧,姜斯佳,冯广旭,王景霞..枳术郁李方对脾-肠气机升降失调便秘小鼠的通便机制研究[J].世界中医药,2024,19(15):2260-2264,5.基金项目
国家重点研发计划中医药现代化研究重点专项(2018YFC1706803)——基于调节"环核苷酸-内分泌-细胞因子网络系统"研究白芍养肝阴的物质基础与作用机制 （2018YFC1706803）
