色谱2024，Vol.42Issue(9)：837-855,19.DOI:10.3724/SP.J.1123.2023.10021
超高效液相色谱-四极杆-飞行时间质谱法鉴定黄大茶化学成分
Identification of chemical components of large-leaf yellow tea by ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry
摘要
Abstract
Large-leaf yellow tea,a slightly fermented yellow tea that is unique to China,has a stronger hypoglycemic effect than other tea varieties,such as green and black tea.Research on large-leaf yellow tea has focused on its hypoglycemic effect owing to the lack of comprehensive techniques to characterize its chemical components;thus,its development and further promo-tion are limited.Therefore,the development of a reliable analytical method to fully characterize the chemical components of large-leaf yellow tea is urgently required.In this study,a reliable strategy based on the data-acquisition technology of ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q TOF/MS)was established to rapidly screen and analyze the main chemical components of large-leaf yellow tea by combining the in-formation of neutral loss groups and characteristic fragment ions.The chromatographic separa-tion experiments were performed on a Waters ACQUITY UPLC BEH C18 column(100 mm×2.1 mm,1.7 μm)with gradient elution using 0.1%formic acid aqueous solution and acetonitrile as the mobile phases.The flow rate was 0.2 mL/min,the sample volume was 2 μL,and the col-umn temperature was 35℃.The mass spectral information of the components in a large-leaf yellow tea solution was collected using the full-information tandem MS(MSE)technique in pos-itive and negative ion modes.The specific chemical components of large-leaf yellow tea was identified as follows.First,a self-established database of tea chemical components was con-structed based on the literature.The mass spectral cleavage pathways of different types of com-pounds in large-leaf yellow tea were then sorted using reference substances,and the character-istics of the fragment ions and neutral loss groups were summarized.The precise mass-to-charge ratio of the target chemical components were then obtained based on the mass spectral information.Finally,the structures of the compounds in large-leaf yellow tea were confirmed based on their chromatographic retention times,mass spectral cleavage pathways,characteris-tic fragment ions,and neutral loss groups.A total of 87 chemical components,including 10 cat-echins,32 flavonoids,16 phenolic acids,12 tannins,6 theaflavins,and 11 compounds in other classes,were identified in large-leaf yellow tea.Representative compounds of various classes,including gallocatechin gallate,quercetin,vitexin,gallic acid,chlorogenic acid,1,3,6-tri-O-galloyl-β-D-glucose,and theaflavin,were selected,and their characteristic fragment ions and neutral loss groups were investigated in detail to reveal the cleavage pathways of different types of compounds in large-leaf yellow tea.The UPLC-Q TOF/MS method established in this study can comprehensively identify the main chemical components of large-leaf yellow tea in a simple,highly sensitive,stable,and reliable manner.This study provides a scientific basis and data sup-port for the discovery of functional ingredients and quality evaluation of large-leaf yellow tea.关键词
超高效液相色谱-四极杆-飞行时间质谱/中性丢失/特征碎片离子/黄大茶/化学成分Key words
ultra performance liquid chromatography-quadrupole time-of-flight mass spectrom-etry(UPLC-Q TOF/MS)/neutral loss/characteristic fragment ions/large-leaf yellow tea/chemical components分类
化学化工引用本文复制引用
黄若童,戎雪雯,付晓洁,陈畅,储俊,许娜,吴欢..超高效液相色谱-四极杆-飞行时间质谱法鉴定黄大茶化学成分[J].色谱,2024,42(9):837-855,19.基金项目
国家自然科学基金(81903765) （81903765）
安徽农业大学茶树生物学与资源利用国家重点实验室开放基金项目(SKLTOF20220105).National Natural Science Foundation of China(No.81903765) （SKLTOF20220105）
The Open Fund of State Key Laboratory of Tea Plant Biology and Utilization,Anhui Agricultural University(No.SKLTOF20220105). （No.SKLTOF20220105）
