水生生物学报2024，Vol.48Issue(9)：1459-1472,中插1-中插2,16.DOI:10.7541/2024.2024.0043
饲料精氨酸含量对海水驯化虹鳟的渗透调节、抗氧化和免疫力的影响
DIETARY ARGININE CONTENT ON OSMOREGULATION,ANTIOXIDANT STATUS,AND IMMUNE CAPACITY OF RAINBOW TROUT(ONCORHYNCHUS MYKISS)DURING SEAWATER ACCLIMATION
摘要
Abstract
This study aimed to investigate the effects of dietary supplementation with arginine(Arg)on osmoregulation,antioxidant status,and immune capacity of rainbow trout Oncorhynchus mykiss before and after seawater acclimation.A total of 480 rainbow trout(initial weight of 67.04±6.93 g)were randomly divided into four groups.Four experimen-tal diets were formulated to contain Arg level of 1.46%(A-1.46),2.50%(A-2.50),3.45%(A-3.45),and 4.62%(A-4.62),respectively.After five weeks of freshwater culture,each group of rainbow trout underwent seawater acclima-tion for 2 days and 4 days,designated as SA-2 and SA-4,respectively.SA-2 entails a gradual increase in salinity from 0 to 15 on the first day,followed by reaching the local seawater salinity of 27 on the second day.SA-4 followed a similar increase as SA-2 on the first day,then increased by 4 units per day until reaching as a salinity of 27.Samples were collected at the conclusion of freshwater culture and at 1d,7d,and 21d after seawater transfer to evaluate osmoregula-tion,antioxidant,and immune parameters.Results showed that at the end of freshwater culture,the A-4.62 group had significantly lower final body weight and percent weight gain,indicating that excessive dietary Arg hinder their growth.Under both acclimation conditions,serum K+levels in the A-3.45 and A-4.62 groups initially increased and then normalized over time,suggesting effective ion regulation in these Arg groups.After 21d after seawater transfer,the liver total antioxidant capacity(T-AOC)of all groups significantly surpassed their respective freshwater values,with the A-3.45 group displaying the lowest integrated biological response(IBR),indicating reduced stress in this group.Compared to the SA-2 method,rainbow trout in all SA-4 groups maintained more stable osmotic pressure,suggesting SA-4's superiority in maintaining osmotic balance.Additionally,under SA-2,the A-3.45 group excreted excess Na+to better adapt in seawater;serum lysozyme(LZM)levels notably rose over time in the A-3.45 group,indicating an enhanced immune response in these rainbow trout after seawater transfer under SA-2.Under SA-2,after 21d of seawa-ter transfer,the A-3.45 group exhibited reduced gill NKA activity to decrease Cl-influx,thereby better maintaining ion balance.In summary,under both acclimation conditions,rainbow trout demonstrated enhanced growth and adaptability when dietary Arg content was 3.45%.关键词
精氨酸/海水驯化/渗透调节/抗氧化能力/免疫力/虹鳟Key words
Arginine/Seawater acclimation/Osmoregulation/Antioxidant status/Immune capacity/Oncorhynchus mykiss分类
农业科技引用本文复制引用
孙云霞,周演根,周思岐,董暄,李丽,高勤峰,董云伟..饲料精氨酸含量对海水驯化虹鳟的渗透调节、抗氧化和免疫力的影响[J].水生生物学报,2024,48(9):1459-1472,中插1-中插2,16.基金项目
国家自然科学基金(32373104) （32373104）
山东省重点研发计划(2021SFGC0701) （2021SFGC0701）
海南热带海洋学院人才科研启动项目(RHDRCZK202401)资助[Supported by the National Science Foundation of China(32373104) （RHDRCZK202401）
the Key Research and Development Program Pro-jects in Shandong Province(2021SFGC0701) （2021SFGC0701）
Research Foundation of Hainan Tropical Ocean University(RHDRCZK202401)] （RHDRCZK202401）
