水生生物学报2024，Vol.48Issue(9)：1473-1482,10.DOI:10.7541/2024.2024.0110
高比例的棉粕和菜粕饲料中添加齐墩果酸对草鱼生长、血清生化指标、抗氧化及免疫力的影响
DIETARY OLEANOLIC ACID ON THE GROWTH PERFORMANCE,SERUM BIOCHEMICAL,ANTIOXIDANT CAPACITY,AND IMMUNITY OF GRASS CARP FED HIGH COTTONSEED AND RAPESEED MEAL DIETS
摘要
Abstract
The aim of this experiment was to investigate the effects of oleanolic acid supplementation in high cotton rapeseed meal feed on the growth,serum biochemistry,antioxidant,and immunity of grass carp(Ctenopharyngodon idella).Four hundred and eighty grass carp weighing about 50 g were selected and randomly divided into three groups with four replicates of 40 fish each.These groups included the control group,the high cotton rapeseed meal group,and the high cotton rapeseed meal group supplemented with 500 mg/kg oleanolic acid,which were labeled as CON,S1,and SQ2,respectively,with a culture period of 56d.The results showed that:(1)Grass carp in the S1 and SQ2 groups exhi-bited significantly lower FBW,WGR,and SGR compared with the CON group(P＜0.05),with the S1 group showing significantly higher FCR(P＜0.05).(2)FBW,WGR,and SGR were significantly higher(P＜0.05),and FCR was signifi-cantly lower(P＜0.05)in grass carp in the SQ2 group compared to the S1 group.Additionally,compared to the CON group,serum GOT,TBA,T-CHO,and hepatic MDA levels were significantly higher(P＜0.05)in the S1 and SQ2 groups.Conversely,levels of serum ACP,IgM,and hepatic CAT,SOD,and GSH were significantly decreased(P＜0.05).Furthermore,serum LDL-C levels were significantly higher(P＜0.05)in the S1 group,whereas serum complement C3 and liver T-AOC levels were significantly lower(P＜0.05).Compared to the S1 group,the SQ2 group exhibited significantly lower levels of serum GOT,T-CHO,and hepatic MDA(P＜0.05),and significantly higher levels of serum ACP,IgM,and hepatic CAT,SOD,and T-AOC(P＜0.05).(3)Compared to the CON group,hepatic expres-sion levels of gpx1,cat,tgf-β1,and il-15 were significantly down-regulated(P＜0.05).Moreover,in the livers of grass carp in S1 group,expression levels of Mnsod and nrf2 were significantly down-regulated(P＜0.05),whereas the expres-sion levels of keap1,tnf-α,nrf-κb.and il-12β were significantly up-regulated(P＜0.05).Conversely,expression levels of Mnsod,gpx1,cat,nrf2,tgf-β1,and il-15 were significantly up-regulated(P＜0.05),while the expression levels of keap1,tnf-α,nf-κb,and il-12β were significantly down-regulated(P＜0.05)in the livers of the grass carp in the SQ2 group compared to the S1 group.In conclusion,the high percentage of cotton rapeseed meal in the feed significantly reduced the growth performance,antioxidant capacity,and immunity of grass carp,and induced damage to the liver tissue struc-ture.However,supplementation with oleanolic acid alleviated oxidative damage and inflammatory responses in the liver of grass carp,while improving growth performance,antioxidant capacity,and immunity.关键词
齐墩果酸/抗氧化能力/免疫力/草鱼Key words
Oleanolic acid/Antioxidant indexes/Immunity/Ctenopharyngodon idella分类
农业科技引用本文复制引用
王绍,胡毅,蔡明浪,戴济鸿,徐树德,胡海波,符贵红..高比例的棉粕和菜粕饲料中添加齐墩果酸对草鱼生长、血清生化指标、抗氧化及免疫力的影响[J].水生生物学报,2024,48(9):1473-1482,10.基金项目
国家自然科学基金(32172985)资助[Supported by the National Natural Science Foundation of China(32172985)] （32172985）
