水生生物学报2024，Vol.48Issue(9)：1502-1508,7.DOI:10.7541/2024.2023.0365
高糖饲料对草鱼葡萄糖耐受的影响
HIGH CARBOHYDRATE FEED ON GLUCOSE TOLERANCE IN GRASS CARP(CTENOPHARYNGODON IDELLA)
摘要
Abstract
To investigate the impact of a high-carbohydrate diet on glucose tolerance in grass carp,a total of 48 common grass carp and 48 grass carp fed with high carbohydrate diet,with an average weight of 374 g,were selected for a glucose tolerance test.The experimental fish were divided into two groups:a control group and a high carbohy-drate group.The injection dosage was determined as 1.5 grams per kilogram of body weight,dissolved in 1 mL of PBS buffer.Additionally,48 grass carp with similar body weight were chosen as the negative control group(PBS group)and injected with 1 mL of PBS buffer.Following the injection,blood glucose and insulin levels were measured at 0,1h,3h,6h,and 12h,and liver samples were collected at the corresponding times to detect the expression of glucose-metabolism related genes.The blood tests showed that the high carbohydrate group exhibited the lowest blood glucose levels at the 0 hour.At the 1st hour,both groups reached peak blood glucose levels,with the high carbohydrate group demonstrat-ing significantly lower levels than the control group.At the 12th hour,blood glucose levels in both groups returned to the normal range.Although insulin levels showed a slight increase post-glucose injection,the difference was not signifi-cant.RT-qPCR results showed that the expression levels of glut2(glucose transporter),g6pase(glucose-6-phosphatase G-6-pase),and gys2(glycogen synthase 2)genes were higher in the carbohydrate group compared to the control group at the 0h mark.Between the 1st and 3rd hours,expression of the gk(glucokinase)and gys2 gene in the high carbohy-drate group was significantly higher than that in the control group.In summary,grass carp fed a high carbohydrate diet exhibited enhanced blood glucose clearance ability and improved glucose tolerance.The primary mechanism underly-ing rapid blood sugar clearance in the high-carbohydrate group grass carp appears to be the enhancement of glycogen synthesis.关键词
高糖饲料/肝脏糖代谢/葡萄糖耐受/草鱼Key words
High carbohydrate feed/Liver glucose metabolism/Glucose tolerance/Ctenopharyngodon idella分类
农业科技引用本文复制引用
朱涛,陈志钊,雷彩霞,杜金星,宋红梅,李胜杰..高糖饲料对草鱼葡萄糖耐受的影响[J].水生生物学报,2024,48(9):1502-1508,7.基金项目
中国水产科学研究院珠江水产研究所基本科研业务费项目(2023SJXT1) （2023SJXT1）
广东省淡水水产种质资源库运行项目(2022-SBH-00-001) （2022-SBH-00-001）
国家淡水水产种质资源库珠江分库(FGRC18537)资助[Supported by the Central Public-interest Scientific Institution Basal Research Fund,CAFS(2023SJXT1) （FGRC18537）
Guangdong Rural Revitalization Strategy Special Provincial Organization and Imple-mentation Project Funds(2022-SBH-00-001) （2022-SBH-00-001）
National Freshwater Genetic Resource Center(FGRC18537)] （FGRC18537）
