水生生物学报2024，Vol.48Issue(9)：1528-1540,13.DOI:10.7541/2024.2024.0103
大菱鲆IGF结合蛋白的营养调控功能
NUTRITIONAL REGULATION OF IGF-BINDING PROTEIN IN TURBOT(SCOPHTHALMUS MAXIMUS L.)
摘要
Abstract
To investigate the impact of IGF-like growth factor binding protein(IGFBP)on the physiological growth and nutritional metabolism of aquatic animals,turbot muscle fibroblasts were chosen as the research subject.The amino acid sequence of igfbp gene in zebrafish(Danio rerio),Homo sapiens(Homo sapiens)and turbot(Scophthalmus maximus L.)was collectively analyzed through bioinformatics to infer their evolutionary history.Furthermore,turbot muscle fibroblasts were treated with IGFBPs inhibitor(NBI 31772)and IGF1R inhibitor(BMS 554417),followed by analysis using fluorescence quantitative PCR,protein western blotting,Annexin V-FITC staining,and metabolite deter-mination.In this study,we investigated the IGFBP family in turbot,identifying 12 genes with distinct tissue expression patterns.Our findings reveal significant tissue-specific expression of different igfbp genes.Specifically,igfbp2a exhi-bited the highest expression in brain,gill,and gallbladder tissues,while igfbp5a displayed peak expression in the eye,muscle,kidney,and skin.Moreover,igfbp3b showed the highest expression in spleen and heart tissue,igfbp2b in the liver,and igfbp4 in intestinal tissues.Additionally,our study sheds light on the dynamic response of IGFBP expression to nutritional induction in turbot muscle fibroblasts.The expression levels of igfbp1a,igfbp3b,igfbp4,igfbp5a,and igfbp5b in turbot muscle fibroblasts decreased first and then increased after nutritional induction.Moreover,our results indicate that igf2 displays greater sensitivity to nutritional stimuli compared to igf1.We also investigated the effects of short-term and long-term inhibition of IGFBPs on IGF signaling.Short-term inhibition promoted IGF signaling,while long-term inhibition resulted in reduced IGF activity and apoptosis.In addition,prolonged IGFBP inhibition led to decreased glycolysis and tricarboxylic acid cycle metabolite levels,alongside intracellular accumulation of free amino acids.In summary,these results provide valuable insights into the role of IGFBPs and the IGF signaling pathways in aquatic animals,offering a foundation for further exploration in this field.关键词
胰岛素样生长信号通路/胰岛素样生长因子结合蛋白/细胞凋亡/营养代谢/大菱鲆Key words
Insulin-like growth signaling pathway/Insulin-like growth factor binding protein/Cell apoptosis/Nutrient metabolism/Scophthalmus maximus L.分类
生物科学引用本文复制引用
曾欢,唐明军,刘成栋,王旋,周慧慧,麦康森,何艮..大菱鲆IGF结合蛋白的营养调控功能[J].水生生物学报,2024,48(9):1528-1540,13.基金项目
国家自然科学基金(32002365) （32002365）
国家重点研发计划(2023YFD2400601) （2023YFD2400601）
国家海水鱼产业技术体系(CARS-47-G10)资助[Sup-ported by the National Natural Science Foundation of China(32002365) （CARS-47-G10）
the National Key Research and Development Program of China(2023YFD2400601) （2023YFD2400601）
the China Agriculture Research System for Marine Fish Culture Industry(CARS-47-G10)] （CARS-47-G10）
