实用医学杂志2024，Vol.40Issue(17)：2369-2374,6.DOI:10.3969/j.issn.1006-5725.2024.17.003
年轻干细胞抗原-1阳性骨髓干细胞调控年老小鼠心脏成纤维细胞凋亡的分子机制
Molecular mechanism of young Sca-1 bone marrow stem cell on old cardiac fibroblast cell apoptosis in aging mice
摘要
Abstract
Objective To investigate the impact of Sca-1 bone marrow derived stem cells on apoptosis in murine cardiac fibroblasts and the molecular mechanisms of young(Y)Sca-1 bone marrow stem cell(BMSC)on old(O)cardiac fibroblast cell(CFC)apoptosis.Methods The apoptosis and survival of Y and O CFC were assessed under hypoxic conditions.Co-cultures of Y and O Sca-1 bone marrow-derived mesenchymal stem cells(BMSC)with O CFC were established to investigate the impact of Sca-1 BMSC on the apoptotic response and viability of O CFC,employing TUNEL staining,qRT-PCR,Western Blot,and CCK8 assays.Furthermore,differential secretion profiles of growth factors by Y and O Sca-1 BMSC were compared using qRT-PCR and ELISA analysis.Results Compared to Y CFC,O CFC exhibited an increased rate of apoptosis and a decreased rate of cell survival.However,when compared to O cells,Y Sca-1 BMC significantly reduced apoptosis in O CFC and enhanced cell survival.Moreover,Y Sca-1 BMSC demonstrated a higher secretion of GDF5(Growth Differentiation Factor 5)than O cells(P<0.05).Importantly,the protective effects of Y Sca-1 BMSC on apoptosis and survival in O CFC were abolished upon neutral-ization of GDF5 expression.Conclusion Y Sca-1 BMSC decreases O CFC apoptosis through GDF5.关键词
干细胞/心脏成纤维细胞/凋亡/细胞存活/生长因子/年轻干细胞抗原-1Key words
stem cell/cardiac fibroblast cell/apoptosis/cell survival/growth factors/Sca-1分类
医药卫生引用本文复制引用
吕饶,于佳迪,李柳蓁,湛楚蓝,赵立越,李月亮,董珺,黎佼..年轻干细胞抗原-1阳性骨髓干细胞调控年老小鼠心脏成纤维细胞凋亡的分子机制[J].实用医学杂志,2024,40(17):2369-2374,6.基金项目
广东省基础与应用基础研究基金项目(编号:2023A1515011721,2024A1515011439) （编号:2023A1515011721,2024A1515011439）
广州医科大学附属第六医院,(清远市人民医院)开放课题基金项目(编号:202201-304) （清远市人民医院）
广州医科大学附属第二医院临床研究项目(编号:2021-LCYJ-DZX-03,2022-LCYJ-YYDZX-03) （编号:2021-LCYJ-DZX-03,2022-LCYJ-YYDZX-03）
广州医科大学2022年度学生创新能力提升计划项目(编号:广医大发[2022]66号) （编号:广医大发[2022]66号）
