miR-223通过调控Keap1/Nrf2/ARE信号通路对前列腺癌细胞损伤的影响OA北大核心CSTPCD
Effects of miR-223 on prostate cancer cell damage by regulating Keap1/Nrf2/ARE signaling pathway
目的 探究微小 RNA-223(miR-223)通过调控Kelch 样环氧氯丙烷相关蛋白 1(Keap1)/核因子E2相关因子 2(Nrf2)/抗氧化反应元件(ARE)信号通路对前列腺癌细胞损伤的影响.方法 培养前列腺癌细胞株PC3,且随机将其分为对照组、下调miR-223组、上调miR-223组.探究miR-223表达、细胞增殖率、细胞迁移数、细胞侵袭数、凋亡率、Keap1/Nrf2/ARE信号通路表达量的变化.结果 与对照组比较,下调miR-223 组的细胞侵袭数、细胞迁移数、24、48、72 h细胞增殖率、Nrf2、ARE表达上升,miR-223、Keap1、凋亡率表达降低(P<0.05);与下调miR-223组比较,上调miR-223组的24、48、72 h细胞增殖率、细胞侵袭数、细胞迁移数、ARE、Nrf2表达下降,miR-223、凋亡率、Keap1表达升高(P<0.05).结论 调节miR-223可有效改善前列腺细胞损伤,其机制可能与Keap1/Nrf2/ARE信号通路有关.
Objective To investigate the effect of microRNA-223(miR-223)on prostate cancer cell damage by regulating the Kelch-like epichlorohydrin related protein 1(Keap1)/nuclear factor E2 related factor 2(Nrf2)/antioxidant response element(ARE)signaling pathway.Methods The prostate cancer cell line PC3 was cultured and randomly divided into control,down-regulated miR-223,and up-regulated miR-223 groups.Changes in miR-223 expression,cell proliferation rate,cell migration number,cell invasion number,apoptosis rate,and expression level of Keap1/Nrf2/ARE signaling pathway were explored.Results Compared with the control group,the cell invasion number,cell migration number,cell proliferation rate,Nrf2 and ARE expression increased at 24,48 and 72 h in down-regulated miR-223 group,while the expressions of miR-223,Keap1 and apoptosis rate decreased(P<0.05).Compared with the down-regulated miR-223 group,24,48 and 72 h cell proliferation rate,cell invasion number,cell migration number,ARE and Nrf2 expression decreased in the up-regulated miR-223 group,while miR-223,apoptosis rate and Keap1 expression increased(P<0.05).Conclusion Regulation of miR-223 effectively ameliorates prostate cell injury,and the mechanism may be related to the Keap1/Nrf2/ARE signaling pathway.
王之仕;李桂凌;陈劲果;王宏
海南省中医院泌尿外科(海口 570203)海南医学院第一附属医院儿科(海口 570102)
基础医学
前列腺癌微小RNA-223Kelch样环氧氯丙烷相关蛋白1/核因子E2相关因子2/抗氧化反应元件细胞损伤
prostate cancermicroRNA-223Kelch-like epichlorohydrin associated protein 1/nuclear factor E2 related factor 2/antioxidant response elementcell injury
《实用医学杂志》 2024 (017)
2375-2380 / 6
海南省自然科学基金项目(编号:823QN356)
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