实用医学杂志2024，Vol.40Issue(17)：2412-2417,6.DOI:10.3969/j.issn.1006-5725.2024.17.010
系统性红斑狼疮患者外周血单个核细胞miR-4524a-3p的表达及临床意义
Expressions and clinical significance of miR-4524a-3p in peripheral blood mononuclear cells of patients with systemic lupus erythematosus
摘要
Abstract
Objective To analyze the expression of miR-4524a-3p in peripheral blood mononuclear cells(PBMCs)and major immune cell subsets of patients with systemic lupus erythematosus(SLE),and evaluate its clinical application.Methods Peripheral blood samples were collected from SLE patients and healthy controls,followed by isolation of PBMCs,CD3+T lymphocytes,CD19+B lymphocytes,and CD14+monocytes using human peripheral blood lymphocyte isolation solution(Ficoll)and immunomagnetic bead cell sorting.The expression level of miR-4524a-3p was quantified using fluorescence quantitative PCR.Pearson's linear correlation analysis was per-formed to assess the relationship between miR-4524a-3p expression and the disease activity score in SLE patients(SLEDAI).Additionally,the diagnostic efficacy of miR-4524a-3p for SLE was evaluated by constructing a receiver operating characteristic curve(ROC).Results The expression of miR-4524a-3p was significantly decreased in PBMCs(P<0.05),CD3+T cells(P<0.001),and CD14+monocytes(P<0.001)from patients with SLE compared to healthy controls.Furthermore,it exhibited a negative correlation with SLEDAI score(r=-0.406,P<0.01).Notably,the expression level of miR-4524a-3p was significantly lower in patients with moderate or severe active stage than those in mild and inactive stages(P<0.01).In addition,the SLE subgroup with lupus nephritis and joint involvement displayed lower levels of miR-4524a-3p compared to the subgroup without such manifestations(P<0.01).Importantly,ROC analysis demonstrated that miR-4524a-3p holds promising diagnostic value for SLE.Conclusion The expression of miR-4524a-3p was significantly diminished in CD3+T cells and CD14+monocytes derived from patients with SLE,exhibiting a close association with disease activity and demonstrating potential clinical diagnostic value for SLE.关键词
系统性红斑狼疮/微小RNA/miR-4524a-3p/临床意义Key words
systemic lupus erythematosus/microRNA/miR-4524a-3p/clinical value分类
社会科学引用本文复制引用
马茜,周少岚,陈会鹃,王延峰..系统性红斑狼疮患者外周血单个核细胞miR-4524a-3p的表达及临床意义[J].实用医学杂志,2024,40(17):2412-2417,6.基金项目
宁夏自然科学基金项目(编号:2021AAC05017,2020AAC03116) （编号:2021AAC05017,2020AAC03116）
