宿州学院学报2024，Vol.39Issue(6)：33-38,6.DOI:10.3969/j.issn.1673-2006.2024.06.007
HPLC法测定颍半夏中10种成分的含量及热图聚类分析
Simultaneous Determination of Ten Components of Pinelliae Ternata in Yingshang by Dual Wavelength Switching Method Heat Map Clustering Analysis
摘要
Abstract
A dual wavelength method was established of HPLC for the determination of 10 components in Pinelliae Ternata in Yingshang,and the difference of 10 components in cultivated and wild Pinelliae Ternata in Yingshang was analyzed by heat map cluster.The chromatographic conditions are as follows:Shiseido C18 column(4.6 mm×250 mm,5 μm)was used.Gradient elution was performed with acetonitrile(A)-0.01%phosphoric acid(B)as mobile phase.The flow rate was 1.0 mL/min.Dual wavelength detection was adopted;Column temperature:30℃;the injection volume was 15 μL.The result shows that the separation effect of 10 components of Pinelliae Ternata in Yingshang was better.Methodological investigation meets relevant requirements.The results of heat map clustering showed that wild Pinelliae Ternata had certain environmental clustering characteristics,with a higher content of tar-get components in it.The establishment of this method could provide a basis for quality evaluation and cultivation base selection of Pinelliae Ternata in Yingshang.关键词
颍半夏/琥珀酸/葫芦巴碱/核苷/HPLCKey words
Pinelliae Ternata in Yingshang/Succinic acid/Trigonelline/Nucleoside/HPLC分类
医药卫生引用本文复制引用
邢亚东,杨兵,丁琪,罗凯文..HPLC法测定颍半夏中10种成分的含量及热图聚类分析[J].宿州学院学报,2024,39(6):33-38,6.基金项目
安徽省自然科学项目(1908085QH376) （1908085QH376）
安徽省中医药传承创新科研项目(2020ccyb20) （2020ccyb20）
安徽省大学生创新创业训练计划项目(S202110367085) （S202110367085）
蚌埠医学院自然科学重点项目(2021byzd034). （2021byzd034）
