土壤学报2024，Vol.61Issue(5)：1374-1385,12.DOI:10.11766/trxb202303090096
长期秸秆掩埋配施氮肥对土壤细菌群落特征的影响
Effects of Long-term Straw Burying and Nitrogen Fertilizer Application on Soil Bacterial Community Characteristics
摘要
Abstract
[Objective]In order to reveal the response mechanism of microbial community and ecological network to straw returning process in typical fluvo-aquic soil,we experimented with different straw-returning treatments under long-term wheat-maize rotation.[Method]The high-throughput sequencing and ecological network methods were utilized to analyze the soil bacterial community composition,bacterial network co-occurrence and their relationships with soil nutrient concentrations.[Result]The results indicated that compared to straw removal and no nitrogen fertilizer treatment,straw returning with conventional fertilization treatments significantly reduced soil pH,while increasing the content of TN,SOC,AP,AK and NO 3--N(P<0.05).The treatments of straw burying with nitrogen fertilizers were beneficial for increasing soil nutrient content.Moreover,no significant difference in bacterial alpha diversity was observed between different straw-returning methods and different amounts of nitrogen fertilizers,but a significant difference was observed in bacterial community structure.Factors such as pH,SOC,and TN drove variations in bacterial community structure.Also,Acidobacteriota,Proteobacteria,Bacteroidota and Chloroflexi were the dominant phyla in the fluvo-aquic soil.Furthermore,co-occurrence network analysis revealed four main ecological clusters that were significantly correlated with soil nutrients.The abundances of taxa in module 1 were found to be inversely correlated with SOC,TN,TP,NO 3--N,AP and AK(P<0.001),and positively correlated with pH(P<0.001).Conversely,the abundances of taxa in module 2 and module 3 were significantly positively correlated with most nutrient content,and negatively related to pH.[Conclusion]Therefore,it can be concluded that straw burying combined with nitrogen fertilizers can improve soil nutrient by regulating ecological relationships of microorganisms.The findings of this study can provide a scientific basis for the efficient utilization of straw and the efficient management of soil fertilization.关键词
秸秆还田/土壤有机碳/细菌群落/网络分析Key words
Straw returning/Soil organic carbon/Bacterial community/Network analysis分类
农业科技引用本文复制引用
刘明凤,周桂香,张佳宝,张丛志,薛载琦,赵占辉..长期秸秆掩埋配施氮肥对土壤细菌群落特征的影响[J].土壤学报,2024,61(5):1374-1385,12.基金项目
国家重点研发计划项目(2022YFD1500903,2022YFD1500502)、安徽省水利水电勘测设计研究总院有限公司技术服务项目和现代农业产业技术体系专项资金(CARS-03,CARS-52)资助 Supported by the National Key R&D Program of China(Nos.2022YFD1500903,2022YFD1500502),the Technical Service Project of Anhui Provincial Water Resources and Hydropower Survey,Design and Research Institute Co.,Ltd,and the Earmarked Fund for CARS(Nos.CARS-03,CARS-52) （2022YFD1500903,2022YFD1500502）
