土壤与作物2024，Vol.13Issue(3)：381-389,9.DOI:10.11689/sc.2023100603
昆虫病原线虫共生菌及代谢产物对根结线虫的防治作用
Control effect of symbiotic bacteria and their metabolites from entomopathogenic nematode on Meloidogyne spp.
摘要
Abstract
In order to assess the control effects of symbiotic bacteria of different species entomopathogenic nematode(EPN)on Meloidogyne spp.as well as the metabolites of those symbiotic bacteria on egg hatching and larval activity of root-knot nematodes,we conducted a pot experiment with quantitative inoculation in greenhouse and a bioassay test in laboratory.The results showed that symbiotic bacteria of entomopathogenic nematode Xb-IGA and Pl-IGA exhibited good suppressive effect on the reproduction of M.incognita eggs,and the inhibition rate ranged 59.4%～73.9%when M.incognita was inoculated with 1 000,2 000 and 5 000 eggs·plant-1 on Zhongshu 4 tomato.When M.hapla was inoculated with 2 000 eggs·plant-1 on VFNT tomato,the inhibition rate of EPN symbiotic bacteria Pl-IGA reached the maximum value of 74.5%,which was not significantly different from the conven-tional control,i.e avermectin(82.2%).Symbiotic bacteria of four species EPN effectively reduced the incidence of M.hapla and M.incognita infection,also promoted the root growth of tomato.After 7 days of bioassay,the 2×and 5×dilutions of metabolites by four species of symbiotic bacteria exhibited significant inhibition on the egg hatching of M.incognita and M.hapla.The corrected mortality of M.incognita larvae was 34.2%～47.8%,and that of M.hapla was 59.6%～79.9%.Therefore,symbiotic bacteria of entomopathogenic nematode and their metabolites showed great potential as biological nematicide resources for controlling root-knot nematode.关键词
根结线虫/昆虫病原线虫/共生菌/代谢产物/防效Key words
root-knot nematode/entomopathogenic nematode/symbiotic bacteria/metabolite/control effect分类
农业科技引用本文复制引用
吴黎黎,赵亚男,窦悦文,王从丽,李春杰,谢倚帆,常豆豆,黄铭慧,姜野,秦瑞峰,蒋丹,韦柳利,王旋..昆虫病原线虫共生菌及代谢产物对根结线虫的防治作用[J].土壤与作物,2024,13(3):381-389,9.基金项目
中国科学院战略性先导科技专项(A类)(XDA28070305) （A类）
长春市科技创新合作专项(23SH17) （23SH17）
财政部和农业农村部:国家现代农业产业技术体系(CAR-04). （CAR-04）
