西北植物学报2024，Vol.44Issue(8)：1190-1200,11.DOI:10.7606/j.issn.1000-4025.20230710
几丁质和flg22诱导的辣椒幼苗先天免疫生理响应特征
Characteristics of innate immune physiological response induced by chitin and flg22 in pepper seedlings
摘要
Abstract
[Objective]The study aims to examine the characteristics of innate immune physiological re-sponses induced by chitin and flagellin-derived peptide flg22 in pepper seedlings,and to explore the rela-tionship between innate immune physiological responses and pepper multiple resistance to diseases.[Methods]Five local pepper cultivars from Sichuan were used as experimental materials.The disease inde-xes of bacterial wilt and phytophthora blight in pepper seedlings were analyzed.Seedlings were cultured by hydroponics and treated with chitin and flg22 exogenously.Root growth,stomata aperture,callose depo-sition,reactive oxygen species(ROS)accumulation,SOD,CAT activities as well as gene expression un-der different induction times were analyzed.The physiological responses and their relationship with disease resistance were evaluated comprehensively using biostatistics.[Results](1)The lowest disease index of bacterial wilt and phytophthora blight was'Chuanteng No.10'('CT10')cultivar,which was the stron-gest in disease resistance.The highest disease index of bacterial wilt and phytophthora blight was'Tiao-jiao'('TJ')cultivar,which was the weakest in disease resistance.(2)Exogenous chitin and flg22 inhibi-ted the root growth rate of pepper seedlings in five cultivars,induced stomatal closure on detached leaves,promoted callose deposition in cell wall,and increased ROS levels with improved SOD and CAT activities.By calculating the average membership function value of the innate immune physiological response,it showed that the highest was'CT10'and the lowest was'TJ'.The average membership function value has a significant negative correlation with the disease index of bacterial wilt and phytophthora blight.(3)flg22 and chitin induced the expression of genes involved in innate immune response in'CT10',such as CaWRKY22,CaMAPK7,and ChiIV3.[Conclusion]Exogenous flg22 and chitin induce the innate im-mune physiological responses in pepper seedlings,with variations among the varieties.The average mem-bership function of'CT10'is the highest and the multi-resistance level is the best,due to the up-regulated expression of CaWRKY22,CaMAPK7,and ChiIV3.关键词
辣椒/植物先天免疫/病原体相关分子模式/flg22/几丁质/生理响应Key words
Capsicum annuum L./plant innate immunity/PAMPs/flg22/chitin/physiological response分类
农业科技引用本文复制引用
巩雪峰,许艺,宋占锋,陈鑫,陈琼,侯思皓..几丁质和flg22诱导的辣椒幼苗先天免疫生理响应特征[J].西北植物学报,2024,44(8):1190-1200,11.基金项目
国家现代农业产业技术体系四川省创新团队项目(sccxtd-2020-05) （sccxtd-2020-05）
四川省科技计划青年创新团队项目(2021JDT0006) （2021JDT0006）
"十四五"四川省蔬菜育种公关项目(2021YFYZ0022) （2021YFYZ0022）
四川省自然科学基金青年基金项目(2023NSFSC1242) （2023NSFSC1242）
四川省财政自主创新专项项目(2022ZZCX45) （2022ZZCX45）
