西北植物学报2024，Vol.44Issue(8)：1239-1249,11.DOI:10.7606/j.issn.1000-4025.20240111
辣椒MADS-box转录因子CaRIN基因克隆、表达与功能分析
Cloning,expression,and functional analysis of the CaRIN gene,encoding a MADS-box transcription factor from pepper
摘要
Abstract
[Objective]The study aims to explore the expression and function of the RIN gene,encoding a MADS-box,and analyze its impact on the metabolism of carotenoids in pepper.[Methods]Based on the fruit development transcriptome of pepper,the full-length CDS of the MADS-box transcription factor Ca-RIN gene related to chili ripening was cloned.The bioinformatics,expression pattern,subcellular locali-zation,and transcriptional activation activity were examined,and the effects of VIGS-induced CaRIN gene silencing on carotenoid metabolism were investigated.[Results](1)The CDS of CaRIN gene was 732 bp in length.CaRIN gene encoded a protein containing 243 amino acids with a molecular mass of 27.95 kD and an isoelectric point(pI)of 7.06.CaRIN protein had a typical MEF2_like MADS domain,belonging to the MICK type transcription factor.(2)CaRIN gene was mainly expressed in flowers and fruits with tissue specificity.CaRIN was located in the nucleus and had transcriptional activation activity.(3)The promoter of CaRIN gene had several hormone response elements such as ABRE,and exogenous ABA and ethephon accelerated the fruit to turn red and induced the high expression of CaRIN and related genes.(4)After VIGS-induced gene silencing of CaRIN,the expression level of PSY1,CCS,PDS,CRTZ,LCYB,and NCED1,genes involved in carotenoid metabolic pathway,was decreased by 0.27-0.59 times,and the total carotenoid content in fruits(0.379 mg/g)was decreased compared with the control(0.650 mg/g).[Conclusion]CaRIN may be an important regulatory factor of carotenoid metabolism in capsicum fruits.关键词
辣椒/MADS-box转录因子/CaRIN/VIGS/类胡萝卜素Key words
pepper/MADS-box transcription factor/CaRIN/VIGS/carotenoid分类
生物科学引用本文复制引用
段敏杰,杨小苗,李怡斐,黄任中,黄启中,张世才..辣椒MADS-box转录因子CaRIN基因克隆、表达与功能分析[J].西北植物学报,2024,44(8):1239-1249,11.基金项目
重庆市农业科学院绩效激励引导专项项目(cqaas2022jxjl002) （cqaas2022jxjl002）
