西北植物学报2024，Vol.44Issue(8)：1261-1272,12.DOI:10.7606/j.issn.1000-4025.20240120
甘蓝型油菜种子硫代葡萄糖苷含量的QTL定位及候选基因分析
QTL mapping and candidate gene identification of seed glucosinolate content in Brassica napus
摘要
Abstract
[Objective]The study aims to discover the genetic loci and candidate genes of seed glucosinolate content in Brassica napus,and lay a foundation for the cloning of genes involved in glucosinolate metabo-lism and improving the breeding of B.napus.[Methods]Using KN DH populations from four different environments as materials,the QTL mapping of seed glucosinolate content and the candidate genes were analyzed.[Results](1)The variation coefficient of seed glucosinolate content in B.napus was high and stable,which followed the genetic characteristics of quantitative traits.(2)Seven consistent QTLs,inclu-ding cqGC.A9-5,cqGC.A9-7,cqGC.A9-9,cqGC.C2-9,cqGC.C2-10,cqGC.C9-5 and cqGC.C9-6,were environmentally stable QTLs,of which cqGC.A9-5,cqGC.C2-10,and cqGC.C9-5 were major QTLs.(3)Three candidate genes,including BnaA09g05480D,BnaC09g05620D,and BnaC09g05810D,were identi-fied in the interval of the major QTL cqGC.A9-5 and cqGC.C9-5.Based on their annotation,these candi-date genes were involved in the biosynthetic pathway of glucosinolates(the biosynthesis of IAOx and 3-al-kyl malic acid)and transport and distribution of glucosinolates.[Conclusion]Seed glucosinolate content in B.napus was quantitative trait.Three major QTLs for seed glucosinolate content were identified,which might be involved in the synthesis of intermediate products and the transport and distribution of glucosino-lates.关键词
甘蓝型油菜/种子硫苷含量/QTL定位/候选基因Key words
Brassica napus L./seed glucosinolate content/QTL mapping/candidate gene分类
农业科技引用本文复制引用
赵卫国,塔娜,王灏..甘蓝型油菜种子硫代葡萄糖苷含量的QTL定位及候选基因分析[J].西北植物学报,2024,44(8):1261-1272,12.基金项目
陕西高校青年创新团队项目(2023AYTD01) （2023AYTD01）
陕西省大学生创新创业训练计划项目(S202311397010) （S202311397010）
陕西省重点研发计划项目(2020ZDLNY04-01) （2020ZDLNY04-01）
陕西省茶产业协同创新项目(23JY001,23JY003) （23JY001,23JY003）
安康学院高层次人才引进项目(2021AYQDZR12) （2021AYQDZR12）
