广西壮族自治区百色市人群miR-145基因多态性分布特征及其与血脂的关系OA

Objective To investigate the distribution characteristics of microRNA-145(miR-145)gene rs41291957 and rs353291 polymorphisms in Baise city,Guangxi Zhuang Autonomous Region,and to compare their genotype and allele frequencies with other ethnic groups.The distribution differences were further analyzed to analyze the relationship between rs41291957 and rs353291 polymorphisms and blood lipids.Methods A total of 275 healthy people who underwent physical examination and miR-145 gene detection in Health Care Department of Baise People's Hospital from July 2020 to December 2021 were selected as the research objects.The genotypes and gene frequencies of rs41291957 and rs353291 were detected by TaqMan probe single nucleotide polymorphism typing technique,and the blood lipid related indexes were detected by TBA-FX8 Canon automatic biochemical analyzer.The differences in gender and ethnic distribution of rs41291957 and rs353291 polymorphisms,as well as the differences in blood lipid levels of different genotypes were compared by statistical methods.Results There were three genotypes of AA,AG and GG in miR-145 gene rs41291957 and rs353291 in 275 subjects.The geno-type frequency distribution of the two sites conformed to the Hardy-Weinberg genetic equilibrium law,and the differences were not statistically significant(P>0.05),which was representative of the population.Compari-son of rs41291957 and rs353291 polymorphisms between different gender subjects,and the differences were not statistically significant(P>0.05).There were no significant difference in the distribution of rs41291957 and rs353291 polymorphisms between 275 subjects and East Asian population,and the distribution of rs353291 polymorphisms between 275 subjects and European population(P>0.05).The distribution of rs41291957 locus polymorphism in 275 subjects was significantly different from that in European population,and the distribution of rs41291957 and rs353291 locus polymorphism was significantly different from that in African,American and South Asian populations(P<0.05).There were no significant differences in blood lip-id levels among the subjects with three genotypes of rs353291 locus,and there were no significant differences in total cholesterol,triglyceride and low density lipoprotein cholesterol among the subjects with three geno-types of rs41291957 locus(P>0.05).There were significant differences in high-density lipoprotein cholester-ol among the three genotypes of rs41291957(P<0.05).Conclusion The distribution of miR-145 gene rs41291957 and rs353291 polymorphisms in Baise city,Guangxi Zhuang Autonomous Region is not affected by gender,but there are differences in the distribution between different races.The polymorphism of miR-145 gene rs41291957 is closely related to the level of high density lipoprotein cholesterol.