新疆农业科学2024，Vol.61Issue(6)：1301-1309,9.DOI:10.6048/j.issn.1001-4330.2024.06.001
陆地棉4-香豆酸辅酶A连接酶基因Gh4CL30的功能分析
Functional analysis of 4-coumarate:CoA ligase gene Gh4CL30 in upland cotton
摘要
Abstract
[Objective]To preliminarily study the biological function of 4-coumarate-CoA ligase(4CL)family gene gh4cl30,which might provide theoretical basis and genetic resources for cotton plant breeding.[Methods]Virus-induced gene silencing technology and gene editing technology was used to ob-tain gh4cl30-silenced and edited plants and determine the flavone and lignin contents of the gene suppressed and knockout plants to investigate field phenotype,seed size and fiber quality.[Results]Both gene silencing and gene editing of the gene resulted in dwarfing of the plants and a significant reduction of lignin content in the plant stalks.Field phenotypic measurements showed that seed size and fiber length were significantly re-duced in the gene edited plants.[Conclusion]Gh4CL30 affects cotton growth and development mainly through regulating lignin biosynthesis.关键词
陆地棉/4-香豆酸辅酶A连接酶/木质素/基因编辑/株高Key words
Gossypium hirsutum L./4CL/lignin/gene editing/plant height分类
农业科技引用本文复制引用
巩隽铭,熊显鹏,张彩霞,邵东南,程帅帅,孙杰..陆地棉4-香豆酸辅酶A连接酶基因Gh4CL30的功能分析[J].新疆农业科学,2024,61(6):1301-1309,9.基金项目
国家自然科学基金项目(31960438) （31960438）
新疆生产建设兵团国际科技合作项目(2021BC001) （2021BC001）
石河子大学高层次人才科研启动项目(RCZK2021B24) （RCZK2021B24）
石河子市重点领域科技攻关项目(2022NY01) National Natural Science Foundation of China(31960438) （2022NY01）
International S&T Cooperation Project of Xinjiang Production and Con-struction Corps(2021BC001) （2021BC001）
Shihezi University High level Talent Research Launch Project(RCZK2021B24) （RCZK2021B24）
Key Scientific and Technological Projects in Key Areas in Shihezi City(2021NY01) （2021NY01）
