新疆农业科学2024，Vol.61Issue(6)：1310-1317,8.DOI:10.6048/j.issn.1001-4330.2024.06.002
陆地棉GHWAT1-35基因的克隆及亚细胞定位
Cloning and subcellular localization of the GHWAT1-35 gene in Gossypium hirsutum
摘要
Abstract
[Objective]To explore the role of GHWAT1-35 gene in the development of cotton fibers.[Methods]In this study,the fiber at different developmental stages post-anthesis of Gossypium hirsutum va-riety Xi9 were used as materials.The full-length cDNA sequence of the GHWAT1-35 gene was successfully cloned and subjected to bioinformatics analysis,real-time fluorescence quantitative(qRT-PCR)analysis,and subcellular localization.[Results]The length of the GHWAT1-35 gene was 1125 bp,encoding 374 ami-no acids,with a relative molecular weight is 40.23 kDa and a theoretical isoelectric point of 8.74.Protein mul-tiple sequence alignment and construction of a systematic evolutionary tree analysis showed that the GHWAT1-35 protein was most closely related to the Gossypium arboreum.The expression of the GHWAT1-35 gene signif-icantly increased at 15 days after flowering,and subcellular localization predicted that the protein was located on the cytoplasmic membrane.The GHWAT1-35 gene was recombined with the pCAMIA1300-35S-YFP vector to construct a fusion expression vector,which was then transformed into Agrobacterium using the freeze-thaw method and after being injected into tobacco.The GHWAT1-35 protein was observed to be located on the cytoplasmic membrane.[Conclusion]The GHWAT1-35 gene plays an important role in fiber develop-ment,providing a foundation for further exploration of its biological function in Gossypium hirsutum.关键词
陆地棉/WAT1/基因克隆/生物信息学/亚细胞定位Key words
Gossypium hirsutum/WAT1/gene cloning/bioinformatics/subcellular localization分类
农业科技引用本文复制引用
马尚洁,李生梅,杨涛,王红刚,赵康,庞博,高文伟..陆地棉GHWAT1-35基因的克隆及亚细胞定位[J].新疆农业科学,2024,61(6):1310-1317,8.基金项目
国家重点研发计划子课题(2021YFD1900802-4) A sbu-project of National key research and development program(2021YFD1900802-4) （2021YFD1900802-4）
