畜牧兽医学报2024，Vol.55Issue(8)：3276-3287,12.DOI:10.11843/j.issn.0366-6964.2024.08.002
单细胞转录组测序技术在家养动物中的应用
Application of Single-Cell Transcriptome Sequencing Technology in Domesticated Animals
摘要
Abstract
Single-cell transcriptome sequencing technology(scRNA-seq)can identify individual cell types and cell states with high-precision resolution,breaking the dilemma that ordinary transcrip-tome sequencing unable to probe the specific expression characteristics of target cells,and playing an important role in the exploration of various fields,which is now widely used in many fields,such as human and mouse developmental biology,oncology,immunology,complex diseases,in-testinal microbiome and clinical applications.In recent years,scRNA-seq has also carried out some pioneering research in the field of animal husbandry,mainly focusing on animal reproduc-tive performance,embryonic development,analysis of key traits,etc.However,the application of scRNA-seq in other fields remains to be in-depth compared to its application in humans.In this paper,workflow of scRNA-seq and its application in domesticated animals is reviewed,intending to provide a reference for improving the efficiency of scRNA-seq analysis and its innovative appli-cation in domesticated animals.关键词
单细胞/转录组测序/家养动物Key words
single-cell/transcriptome sequencing/domesticated animals分类
农业科技引用本文复制引用
张肖旭,李昊,冯平捷,杨豪,李新月,吕冉,潘章源,储明星..单细胞转录组测序技术在家养动物中的应用[J].畜牧兽医学报,2024,55(8):3276-3287,12.基金项目
国家重点研发计划青年科学家项目(2023YFF1001800) （2023YFF1001800）
国家重点研发计划(2022YFF1000103) （2022YFF1000103）
国家自然科学基金(31802031 （）
31960659) （）
中国农业科学院科技创新工程(CAAS-ZDRW202106 （）
ASTIP-IAS13) （）
财政部和农业农村部:国家现代农业产业技术体系资助(CARS-38) （CARS-38）
