畜牧兽医学报2024，Vol.55Issue(8)：3493-3502,10.DOI:10.11843/j.issn.0366-6964.2024.08.022
基于4D-DIA技术的伊犁马早期妊娠血清蛋白质组分析
Analysis of Serum Proteomics in Early Pregnancy of Yili Horses Based on 4D-DIA Technology
摘要
Abstract
;The purpose of this article was to explore the serum proteomic characteristics of early pregnancy in Yili horses using proteomic techniques,screen for differential proteins,and analyze their roles in pregnancy.In this study,20 healthy Yili mares with the same age and similar weight were selected for artificial insemination after natural estrus.Pregnancy diagnosis was per-formed on the 13th day after ovulation in the mare.Three pregnant mares and three non pregnant mares were selected based on ultrasound results,and their serum samples were collected.Using 4D-DIA technology for protein quantitative and qualitative analysis of serum samples from Yili mares,screening differential proteins,and conducting bioinformatics analysis such as GO and KEGG.The results showed that a total of 823 proteins were identified,with a molecular weight range of 8-1 010 ku;Based on the threshold of absolute multiple of differences greater than 1.5 times and P＜0.05,53 proteins with significant differences were screened,of which 26 proteins were upregulated and 27 proteins were downregulated.Differential proteins mainly participated in biological processes such as cellular metabolism,biological regulation,metabolism,response to stimuli,cellular component organization,or biogenesis;The KEGG enrichment pathways included proteasomes,arginine biosynthesis,PI3K Akt signaling,ECM receptor interactions,and other pregnancy related pathways.Based on biological analysis such as GO and KEGG,six significantly different proteins were preliminarily screened for possible relationship with early pregnancy,and protein interaction network diagrams were drawn using them.The results showed that CTSS,MMP1,SERPINA5,and others were at key nodes.Six pregnancy specific proteins have been preliminarily screened in this study,providing basic data for screening preg-nancy specific proteins and molecular regulatory mechanisms in equine animals in the future.关键词
蛋白质组/伊犁马/妊娠/血清Key words
proteome/Yili horse/pregnancy/serum分类
农业科技引用本文复制引用
曾明敏,姚新奎,孟军,曾亚琦,王建文,邓海峰,任万路,薛宇恒,尚婷婷,高凤..基于4D-DIA技术的伊犁马早期妊娠血清蛋白质组分析[J].畜牧兽医学报,2024,55(8):3493-3502,10.基金项目
新疆维吾尔自治区重大科技专项"国产马专门化品系高效繁育技术体系构建"(2022A02013-1) （2022A02013-1）
自治区创新环境(人才、基地)建设专项"马种质创新培育与高效健康养殖重点实验室平台建设"(PT2311) （人才、基地）
中央引导地方科技发展专项资金项目"专门化运动马培育技术服务支撑"(ZYYD2023C02) （ZYYD2023C02）
