禽白血病病毒J亚群感染鸡组织中细胞受体chNHE1的表达分析OA北大核心CSTPCD

This study aimed to investigate the expression of chicken sodium hydrogen exchanger isoform 1(chNHE1)in different types of chicken tissues,the tissue tropism of subgroup J avian leukosis virus(ALV-J),and the changes in mRNA of chNHE1 after ALV-J infection.We used qPCR to detect the transcription of chNHE1 in the organs of commercial broilers,commercial layers,and SPF chickens,as well as the viral load in various tissues and the changes in chNHE1 transcription in tissues after ALV-J infection.The results showed that chNHE1 was transcripted in the heart,liver,spleen,lung,kidney,cecal tonsil,thymus,and bursa in all three types of chickens,but the transcription levels varied greatly,with relatively higher transcription levels in the immune organs.After SPF chickens were infected with ALV-J,the viral load was relatively higher in the heart,spleen,kidney,and cecal tonsils,while it was relatively lower in the liver,lung,thymus,and bursa.The transcription of chNHE1 in the spleen,kidney,and cecal tonsils was significantly upregulated after ALV-J infection,but there was no significant change in the transcription in the heart compared to the control group.This study analyzed the tissue transcrip-tion characteristics of chNHE1 in three types of chicken and the effect of ALV-J infection on the transcription chNHE1 in tissues,providing an important theoretical basis for exploring the rela-tionship between the transcription of the receptor chNHE1 and the tissue tropism of ALV-J.