畜牧兽医学报2024，Vol.55Issue(8)：3640-3648,9.DOI:10.11843/j.issn.0366-6964.2024.08.035
狐狸流产胎儿体内肺炎克雷伯菌的分离鉴定及耐药性分析
Isolation,Identification and Drug Resistance Analysis of Klebsiella pneumoniae in Aborted Fetuses of Fox
摘要
Abstract
In order to understand the etiology of abortion in foxes,the aborted fetuses in a fox farm in Hebei Province was analyzed by dissection,pathogen isolation and cultivation,16S rRNA sequencing,virulence gene detection,serotype detection,drug susceptibility analysisassay and pathogenicity analysis assay in this study.As a result,6 strains of Klebsiella pneumoniae were i-solated.Twelve virulence genes(wabG,fimH,ent,icuA,icuB,mrkA,ycf,ecp,uge,ure-A,iroB and ybtA)and three serotypes(K20,K54 and K57)were determined,and the K57 was the dominant serotype.Through K-B drug susceptibility testing,it showed that the isolated K.pneumoniae was moderately or highly sensitive to aminoglycosides,cephalosporins,quinolones,polypeptides and other antibacterial drugs,and multiple drug resistance to macrolides,tetracy-cline,penicillin and other antibacterial drugs was found.According to the pathogenicity analysis,the K.pneumoniae isolate had certain pathogenicity to mice,and the median lethal dose(LD50)was 1.2 × 107CFU·mL-1.In general,K.pneumoniae isolate showed certain pathogenicity,drug resistance,and carrying a variety of virulence genes in this study,and it might be one of the factors causing abortion of fox fetuses.The findings might provide hints for prevention and con-trol of fox fetal abortion.关键词
狐狸/肺炎克雷伯菌/毒力基因/分离鉴定Key words
fox/Klebsiella pneumoniae/virulence gene/solation and identification分类
农业科技引用本文复制引用
王奇林,曹润来,王威阳,张博,刘志杰,王晓旭..狐狸流产胎儿体内肺炎克雷伯菌的分离鉴定及耐药性分析[J].畜牧兽医学报,2024,55(8):3640-3648,9.基金项目
国家重点研发计划项目(2023YFD1800700) （2023YFD1800700）
中央级公益性科研院所基本科研业务费专项(1610342023016) （1610342023016）
吉林省发改委项目(2023C029-5) （2023C029-5）
中国农业科学院科技创新工程(CAAS-ASTIP-2021-ISAPS) （CAAS-ASTIP-2021-ISAPS）
