畜牧兽医学报2024，Vol.55Issue(8)：3659-3668,10.DOI:10.11843/j.issn.0366-6964.2024.08.037
基于宏基因组测序对中国北方地区生乳中嗜冷菌的群落结构研究
Study on the Community Structure of Psychrophilic Bacteria in Raw Milk from North Regions of China based on Metagenomic Sequencing
摘要
Abstract
The aim of this study was to investigate the community structure diversity of psychro-philic bacteria in raw milk in northern China by metagenomic sequencing.A total of 14 raw milk samples were collected from 14 pastures in Inner Mongolia and Beijing,China in winter.The cul-turable psychrophilic bacteria were isolated and purified,total microbial DNA in raw milk was extracted as well.Moreover,the psychrophilic bacteria in raw milk were analyzed by 16S rDNA sequencing and metagenomic sequencing,respectively.The results showed that no significant difference was found in community structure of psychrophilic bacteria in raw milk between Beijing and Inner Mongolia.Pseudomonas,Stenotrophomonas and Brevundimonas were the dominant psychrophiles bacteria in raw milk from the two regions.The global and overview profiles,amino acid metabolism and carbohydrate metabolism were the most important functional metabolic pathways,and psychrophilic bacteria contained a variety of antibiotic resistance genes.In conclu-sion,this study used traditional culture and metagenomic sequencing to accurately determine the species and functional information of the psychrophilic community in raw milk,which is helpful to understand the life activities of psychrophilic bacteria in raw milk and provide basic information for risk assessment of psychrophilic bacteria.Furthermore,it lays down foundational knowledge pivotal for the effective prevention and management of pasture-related issues in Beijing and Inner Mongolia,China.关键词
生乳/嗜冷菌/宏基因组测序/地区/结构Key words
raw milk/psychrophilic bacteria/metagenomic sequencing/region/structure分类
轻工纺织引用本文复制引用
许文君,郑楠,王加启,孟璐..基于宏基因组测序对中国北方地区生乳中嗜冷菌的群落结构研究[J].畜牧兽医学报,2024,55(8):3659-3668,10.基金项目
国家重点研发计划(2022YFD1301003) （2022YFD1301003）
农产品质量安全风险评估(GJFP20220304) （GJFP20220304）
国家奶牛产业技术体系(CARS36) （CARS36）
中国农业科学院科技创新工程(ASTIP-IAS12) （ASTIP-IAS12）
