基于宏基因组测序对中国北方地区生乳中嗜冷菌的群落结构研究OA北大核心CSTPCD

The aim of this study was to investigate the community structure diversity of psychro-philic bacteria in raw milk in northern China by metagenomic sequencing.A total of 14 raw milk samples were collected from 14 pastures in Inner Mongolia and Beijing,China in winter.The cul-turable psychrophilic bacteria were isolated and purified,total microbial DNA in raw milk was extracted as well.Moreover,the psychrophilic bacteria in raw milk were analyzed by 16S rDNA sequencing and metagenomic sequencing,respectively.The results showed that no significant difference was found in community structure of psychrophilic bacteria in raw milk between Beijing and Inner Mongolia.Pseudomonas,Stenotrophomonas and Brevundimonas were the dominant psychrophiles bacteria in raw milk from the two regions.The global and overview profiles,amino acid metabolism and carbohydrate metabolism were the most important functional metabolic pathways,and psychrophilic bacteria contained a variety of antibiotic resistance genes.In conclu-sion,this study used traditional culture and metagenomic sequencing to accurately determine the species and functional information of the psychrophilic community in raw milk,which is helpful to understand the life activities of psychrophilic bacteria in raw milk and provide basic information for risk assessment of psychrophilic bacteria.Furthermore,it lays down foundational knowledge pivotal for the effective prevention and management of pasture-related issues in Beijing and Inner Mongolia,China.