畜牧兽医学报2024，Vol.55Issue(8)：3678-3687,10.DOI:10.11843/j.issn.0366-6964.2024.08.039
mdv1-miR-M4-5p对MDCC-MSB1细胞增殖和凋亡的影响
Effects of mdv1-miR-M4-5p Encoded by MDV on Proliferation and Apoptosis of MDCC-MSB1 Cells
摘要
Abstract
The purpose of this experiment was to study the effects of mdv1-miR-M4-5p encoded by Marek's disease virus(MDV)on proliferation and apoptosis of MDCC-MSB1 cells.In this study,mdv1-miR-M4-5p mimics,inhibitors and their negative controls were transfected into MSB1 cells for 48 h.Real-time quantitative fluorescent PCR(qRT-PCR)was used to detect the transcription of mdv1-miR-M4-5p,TGF-β1,Smad2,caspase-9,caspase-3,cyt-c,cyclinD1,Bcl-2 et al other molecules related to proliferation and apoptosis in MDCC-MSB1 cells.Cell pro-liferation was detected by CCK-8 and cell cycle and apoptosis were detected by flow cytometry.The results showed that compared with the control group,the transcription levels of mdv1-miR-M4-5p,cyclinD1 and Bcl-2 in MDCC-MSB1 cells transfected with mdv1-miR-M4-5p mimics was significantly up-regulated.The transcription of TGF-β1,Smad2,cyt-c,caspase-9 and caspase-3 was down-regulated,the proliferation of MDCC-MSB1 cells was promoted,the the number of G1 phase cells were decreased,the S and G2 cells were increased,and the apoptosis rate was decreased.Compared with the control group,the results of the proliferation and apoptosis of MDCC-MSB1 cells and the expression of related molecules transfected with mdv1-miR-M4-5p inhibitor were opposite to those transfected with mdv1-miR-M4-5p mimics.The results showed that Mdv1-miR-M4-5p could promote the proliferation and inhibit the apoptosis of MDCC-MSB1 cells.This may be conducted by inhibiting the TGF-β1/Smad2 signaling pathway.关键词
mdv1-miR-M4-5p/MDV/增殖/凋亡/TGF-β1/Smad2Key words
mdv1-miR-M4-5p/MDV/proliferation/apoptosis/TGF-β1/Smad2分类
农业科技引用本文复制引用
余祖华,高梦茹,何雷,魏颖,陈建,陈松彪,丁轲..mdv1-miR-M4-5p对MDCC-MSB1细胞增殖和凋亡的影响[J].畜牧兽医学报,2024,55(8):3678-3687,10.基金项目
国家自然科学基金(U1504308 （）
31702207) （）
