吉林黄鸡、北京油鸡及其杂交群体LEPR基因多态性与屠宰性能和肉质性状关联分析OA北大核心CSTPCD

In this study,Jilin yellow chickens,Beijing oil chickens,and their ortho-and trans-breeding heterozygous populations were used as the research objects in order to investigate the genetic diversity of the LEPR gene in local chickens and the correlation of the diversity with the production traits of the chickens.The polymorphic sites of the LEPR gene were excavated by direct sequencing through genome PCR,followed by competitive allele specific PCR(KASP)for genotyping detection,and one-way ANOVA was used for the correlation analysis of slaughtering and meat quality traits of individuals of different genotypes in the different populations.Finally,a one-way analysis of variance(ANOVA)was employed to examine the relationship between the variables related to the meat quality and slaughter performance in the indi-viduals with varying genotypes.The results were as follows:The genetic diversity analysis of the g.427 C＞T and g.735 G＞A loci by KASP showed that T∶T was the major genotype of g.427 C＞T in Jilin yellow chickens(HH)and C∶C in Beijing oil chickens;and A∶G was the main genotype of g.735 G＞A in all the four groups.Three SNPs loci were found in intron 2 of the LEPR gene,of which g.735 G＞A was con-sistent with the report.All the four populations had the predominant genotype A∶G,and all except the HH population had Hardy-Weinberg disequilibrium at the g.427 C＞T locus,whereas all the populations showed equilibrium at the g.735 G＞A locus;both the loci were linked to the percentage of abdominal fat,according to the correlation analysis.However,the various genotypes did not significantly differ between the populations;and in the majority of the populations,the various genotypes of the g.735 G＞A locus were also linked to the foot weight and the loss of pectoral and leg muscle drip.To sum up,the examination of the polymorphic loci of the LEPR gene and its association with character-istics related to meat quality and slaughter in the local hens offered a theoretical foundation for the application of this gene in the breeding and selection of premium chickens.