波棱瓜子总木脂素的急性和亚慢性毒性试验OA北大核心CSTPCD

In order to evaluate the safety of total lignans of Semen herpetospermi,the acute toxicity and subchronic toxicity tests were car-ried out in mice and rats,respectively.In the acute transoral toxicity test,observation lasted for 7 days after the administration of a maximum single dose(5 000 mg/kg).In the subchronic toxicity test,40 SD rats were randomly divided into the control group(0.5%sodium carboxy-methyl cellulose),the low(100 mg/kg),medium(200 mg/kg)and high(400 mg/kg)dose groups.The rats were administered by gavage once a day for 35 consecutive days.At the end of the experiment,the body weight,food consumption,blood index,biochemical index,organ coefficient and histopathological changes in the rats were determined.The results showed that,in the acute toxicity test,the LD50 of total lig-nan of Semen herpetospermi in the ICR mice was higher than 5 000 mg/kg.It was suggested that the total lignans was actually non-toxic ac-cording to the classification standard of acute toxicity of exogenous chemicals.For the subchronic toxicity test,there were no significant differ-ences in hematology,coagulation indicators,blood biochemistry,and organ coefficients among the total lignans groups and the control group(P＞0.05).The histopathological examination revealed that there were no significant pathological changes related to the total lignans.These results indicated that total lignans of Semen herpetospermi could be used in veterinary clinic practice at an appropriate dose.